Effect of black okara hydrolyzates on inflammatory factors in glucose-induced human monocytes

• Main Authors: Lim, Swee-Chin, 林瑞晶
• Other Authors: Tim K. Tso
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/10702607284677084377

碩士 === 國立嘉義大學 === 食品科學系研究所 === 99 === Abstract Activation of circulating monocytes by hyperglycemia plays a significant role in inflammation. The main purpose of this study was to identify the health functionality of black okara hydrolyzates by enzymatic hydrolysis of alcalase and protease N. The objective was to study the effect of black okara hydrolyzates on inflammatory factors in high glucose (HG) concentration (Glucose, 25 mM)-induced human monocyte THP-1. Black okara hydrolyzates derived from 2 and 4 hour-hydrolysis of alcalase and protease N were experimental samples and the analyzed inflammatory factors in this study included Nε-(carboxymethyl) lysine (CML), COX-2, ERK1/2, TNF-α and IL-1β. Results of this study showed that black okara hydrolyzates derived from 2 hour-hydrolysis of alcalase and protease N not only inhibited HG-induced production of TNF-α, IL-1β and COX-2 in human monocytes THP-1 but also decreased the glycation index product CML. The finding of this study suggests that black okara hydrolyzates by enzymatic hydrolysis of alcalase and protease N can at least in part play a role in anti-inflammation.