Residual Study of Enrofloxacin in Grouper (Epinephelus coioides) by High Performance Liquid Chromatography with Fluorescence Detector in Grouper

• Main Authors: Yi-Jing Syue, 薛宜靜
• Other Authors: Jiann-Hsiung Wang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/11087485418233892444

碩士 === 國立嘉義大學 === 獸醫學系研究所 === 99 === In this study, grouper with enrofloxacin 20 mg / kg oral once added to the feed. Using HPLC, detected concentrations of enrofloxacin and ciprofloxacin distribution, in grouper liver, kidney, muscle and serum. The maximal detective concentration in grouper’s liver was 1.36μg/mL at the 8th hours post dosing enrofloxacin of 20mg/kg. After 12 hours post dosing, the maximal detective concentration in kidney and muscle was 15.49 μg/mL and 2.04 μg/mL, respectively. After 24 hours post dosing, the maximal detective concentration in serum was 1.06 μg/mL. The maximal detective concentration of ciprofloxacin in grouper’s kidney at the 36 hours was 9.90 μg/mL. After 48 hours post feeding, the maximal detective concentration in liver, muscle and serum was 1.22 μg/mL, 0.49 μg/mL and 1.00 μg/mL, respectively. After 70 days of feeding, could not be detected in liver, kidney, muscle and serum by HPLC. Enrofloxacin and ciprofloxacin could not be detected in water by HPLC with fluorescence detector.