| Summary: | 碩士 === 國立嘉義大學 === 電子物理學系研究所 === 99 === A biosensor is an analytical device for the detection of biomolecular interaction analysis and is helpful to the development of medical appliance. The biosensor has high sensitivity and selectivity with combining photoelectric technology and biochemistry. The technique of surface plasmon resonance (SPR) is commonly used for monitoring the micro-adsorption of biomolecules among numerous biosensors. The SPR-based sensors have many advantages, such as label-free, real time, high sensitivity and accuracy so that it widely applies in many areas.
In this study, we developed a novel SPR biosensor that can acquire the data of full SPR spectrum in real time, called full-SPR-spectrum image sensor. This system was improved from attenuated total reflection (ATR) technique. It was utilized convex lenses to generate a cone-shaped beam and cause the multi-angle of incident light. A dark line which indicates the dip position of the SPR spectrum was shown in the full-SPR-spectrum image. This system is applied to detect the variation of zein protein with TiO2 photocatalyst reaction. By analyzing the data of images, we observed the shift of SPR dip and estimated the various adsorptions.
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