The Study of Mineralization Performance and Mechanics Character for Osteoblasts Seed on the Different Bio-materials

• Main Authors: Zih Ping Cai, 蔡子平
• Other Authors: Chyung Ay, Ph. D.
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/01541933300424416815

碩士 === 國立嘉義大學 === 生物機電工程學系研究所 === 99 === Surface properties of materials can vary cell growth and differentiation. In this study, PDMS, PCL and PLGA were used to study cell adhesive force of osteoblasts on different types of materials. In addition, osteoblasts were cultured in medium with the addition of β-glycerophosphate and ascorbic acid to study the influence on the cell adhesive force. In cell mineralization study, osteoblasts were growth and mineralized on PDMS, PCL, PLGA and PMMA surfaces and AFM was used to measure the material roughness and cell elasticity on materials. Results show that material roughness is PMMA >PLGA>PCL>PDMS. The dielectrophoresis force produced by the driving voltage of electrodes is used to measure osteoblast adhesion strength on the biomaterial surface. In osteoblast adhesive study, the electric adhesion force of osteoblasts show non-linear relationship with time but osteoblasts had best adhesion force after eight hours cultivation. Generally, cell adhesion force on material surface is PLGA>PCL>PDMS and the addition of β-glycerophosphate and ascorbic acid would decrease cell adhesion force. In osteoblast mineralization, we used alizarin red S dye to stain calcium precipitate. The atomic force microscope was used to measure elasticity of osteoblasts based on force - displacement curve. Results showed that calcium content was increased with time. By the measurement of AFM, the elastic modulus of osteoblasts was risen with time. These results suggested that the mineralization of osteoblasts can increase cell elasticity. After 21 days of osteoblast mineralization, the cell behaviors on different types of materials are various. Osteoblasts on PMMA surface showed higher calcium content and elasticity. In conclusion, material roughness can vary osteoblast mineralization.