To investigate the relationship between mutation frequency of RNA-dependent RNA polymerase and viral virulence in influenza A virus replication
碩士 === 國防醫學院 === 微生物及免疫學研究所 === 99 === Influenza A virus belongs to segmented, negative-sense RNA viruses which employ virus-encoded RNA-dependent RNA polymerase (RdRP) in a ribonucleoprotein (RNP) complex. RNP complex formed by PA, PB1, PB2 proteins plus each genome segment coated by NP, to perform...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2011
|
Online Access: | http://ndltd.ncl.edu.tw/handle/22654341593058618590 |
id |
ndltd-TW-099NDMC0380007 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-099NDMC03800072015-10-28T04:07:08Z http://ndltd.ncl.edu.tw/handle/22654341593058618590 To investigate the relationship between mutation frequency of RNA-dependent RNA polymerase and viral virulence in influenza A virus replication 探討A 型流感病毒複製作用中核醣核酸聚合酶的突變頻率與病毒毒力之關聯性 Sen-Yu Wang 王森右 碩士 國防醫學院 微生物及免疫學研究所 99 Influenza A virus belongs to segmented, negative-sense RNA viruses which employ virus-encoded RNA-dependent RNA polymerase (RdRP) in a ribonucleoprotein (RNP) complex. RNP complex formed by PA, PB1, PB2 proteins plus each genome segment coated by NP, to perform viral replication and mRNA transcription in the nuclei of the infected cells. Despite structural proteins HA and NA of influenza viruses have been demonstrated to be the important determinants for host range change and viral virulence, how influenza RdRP per se contributes to alteration of host spectrum and increase of virulence remains not fully understood. It has been demonstrated that the attenuated poliovirus vaccine can be engineered by enhancing its RdRP fidelity to restrict quasi-species diversity. In this study, we setup a novel system that enable us to estimate the accumulative mutation-frequency of influenza virus RdRP replication. We found Swine Origin Influenza Virus (SOIV)、A/WSN/33/H1N1 and A/PR8/34/H1N1 S216G with a low mutation frequency. Therefore, we would like to investigate virulence of influenza A virus (A/WSN/1933(H1N1)) at 216G. The assay system established by this study enable us to further understand the potential role of fidelity and virulence in influenza A virus RdRP replication. Ching-Len Liao 廖經倫 2011 學位論文 ; thesis 66 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 國防醫學院 === 微生物及免疫學研究所 === 99 === Influenza A virus belongs to segmented, negative-sense RNA viruses which employ virus-encoded RNA-dependent RNA polymerase (RdRP) in a ribonucleoprotein (RNP) complex. RNP complex formed by PA, PB1, PB2 proteins plus each genome segment coated by NP, to perform viral replication and mRNA transcription in the nuclei of the infected cells. Despite structural proteins HA and NA of influenza viruses have been demonstrated to be the important determinants for host range change and viral virulence, how influenza RdRP per se contributes to alteration of host spectrum and increase of virulence remains not fully understood. It has been demonstrated that the attenuated poliovirus vaccine can be engineered by enhancing its RdRP fidelity to restrict quasi-species diversity. In this study, we setup a novel system that enable us to estimate the accumulative mutation-frequency of influenza virus RdRP replication. We found Swine Origin Influenza Virus (SOIV)、A/WSN/33/H1N1 and A/PR8/34/H1N1 S216G with a low mutation frequency. Therefore, we would like to investigate virulence of influenza A virus (A/WSN/1933(H1N1)) at 216G. The assay system established by this study enable us to further understand the potential role of fidelity and virulence in influenza A virus RdRP replication.
|
author2 |
Ching-Len Liao |
author_facet |
Ching-Len Liao Sen-Yu Wang 王森右 |
author |
Sen-Yu Wang 王森右 |
spellingShingle |
Sen-Yu Wang 王森右 To investigate the relationship between mutation frequency of RNA-dependent RNA polymerase and viral virulence in influenza A virus replication |
author_sort |
Sen-Yu Wang |
title |
To investigate the relationship between mutation frequency of RNA-dependent RNA polymerase and viral virulence in influenza A virus replication |
title_short |
To investigate the relationship between mutation frequency of RNA-dependent RNA polymerase and viral virulence in influenza A virus replication |
title_full |
To investigate the relationship between mutation frequency of RNA-dependent RNA polymerase and viral virulence in influenza A virus replication |
title_fullStr |
To investigate the relationship between mutation frequency of RNA-dependent RNA polymerase and viral virulence in influenza A virus replication |
title_full_unstemmed |
To investigate the relationship between mutation frequency of RNA-dependent RNA polymerase and viral virulence in influenza A virus replication |
title_sort |
to investigate the relationship between mutation frequency of rna-dependent rna polymerase and viral virulence in influenza a virus replication |
publishDate |
2011 |
url |
http://ndltd.ncl.edu.tw/handle/22654341593058618590 |
work_keys_str_mv |
AT senyuwang toinvestigatetherelationshipbetweenmutationfrequencyofrnadependentrnapolymeraseandviralvirulenceininfluenzaavirusreplication AT wángsēnyòu toinvestigatetherelationshipbetweenmutationfrequencyofrnadependentrnapolymeraseandviralvirulenceininfluenzaavirusreplication AT senyuwang tàntǎoaxíngliúgǎnbìngdúfùzhìzuòyòngzhōnghétánghésuānjùhéméidetūbiànpínlǜyǔbìngdúdúlìzhīguānliánxìng AT wángsēnyòu tàntǎoaxíngliúgǎnbìngdúfùzhìzuòyòngzhōnghétánghésuānjùhéméidetūbiànpínlǜyǔbìngdúdúlìzhīguānliánxìng |
_version_ |
1718113505906786304 |