To investigate the relationship between mutation frequency of RNA-dependent RNA polymerase and viral virulence in influenza A virus replication

• Main Authors: Sen-Yu Wang, 王森右
• Other Authors: Ching-Len Liao
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/22654341593058618590

碩士 === 國防醫學院 === 微生物及免疫學研究所 === 99 === Influenza A virus belongs to segmented, negative-sense RNA viruses which employ virus-encoded RNA-dependent RNA polymerase (RdRP) in a ribonucleoprotein (RNP) complex. RNP complex formed by PA, PB1, PB2 proteins plus each genome segment coated by NP, to perform viral replication and mRNA transcription in the nuclei of the infected cells. Despite structural proteins HA and NA of influenza viruses have been demonstrated to be the important determinants for host range change and viral virulence, how influenza RdRP per se contributes to alteration of host spectrum and increase of virulence remains not fully understood. It has been demonstrated that the attenuated poliovirus vaccine can be engineered by enhancing its RdRP fidelity to restrict quasi-species diversity. In this study, we setup a novel system that enable us to estimate the accumulative mutation-frequency of influenza virus RdRP replication. We found Swine Origin Influenza Virus (SOIV)、A/WSN/33/H1N1 and A/PR8/34/H1N1 S216G with a low mutation frequency. Therefore, we would like to investigate virulence of influenza A virus (A/WSN/1933(H1N1)) at 216G. The assay system established by this study enable us to further understand the potential role of fidelity and virulence in influenza A virus RdRP replication.