| Summary: | 碩士 === 國防醫學院 === 藥學研究所 === 99 === Diyu, the dried root of Sanguisorba officinalis L.(Rosaceae), is used as antidote and hemostatic for the treatment of burns, scalds, and internal hemorrhage in the tradition at Chinese medicine.
The alcohol extracts of Diyu were treated with n-hexane, CH2Cl2, and n-BuOH to give three fractions, respectively. According to their antivirus activity, the CH2Cl2 and n-BuOH fractions were subjected on silica gel and reversed-phase chromatographies to yield five new compounds, SAOF-K07 (1), K15 (2), K16 (3), K19 (4) and K08 (5), together with eighteen known compounds. The known compounds were identified as 2-oxopomolic acid (6), methyl 3-methoxy-4,5-dihydroxybenzoate (7), 4,5-dimethoxy-3-hydroxybenzoic acid methyl ester (8), methyl syringate (9), methyl mycophenolate (10), clavatol (11), trichothecin (12), prunetin (13), 3,3',4′-O-trimethylellagic acid (14), 3,4'-O-dimethylellagic acid (15), ellagic acid-4-O-β-xylopyranoside-3,3',4'-trimethyl ether (16), 3-oxo-19α-hydroxyurs-12-en-28-oic acid (17), euscaphic acid (18), 28-O-β-D-glucopyranosyl-pomolic acid ester (19), ziyu glycoside II (20) , 3β-[(α-L-arabinopyranosyl)oxy]-urs-12,19(29)- dien-28-oic acid 28-β-D-glucopyranosyl ester (21), ziyu glycoside I (22), and 3β-[(α-L-arabinopyranosyl)oxy]-urs-11,13(18)-dien-28-oic acid β-D-glucopyranosyl ester (23) by comparison their spectroscopic data with those reported in the literature. Among them, compounds 6-13 were isolated for the first time from this plant.
In the antiviral screening in vitro, SAOF-K09, K11 showed significant anti-H1N1 and H3N2 activity, respectively. However, SAOF-K07, K15, K19 only exhibited anti-H1N1 activity, and SAOF-K01 showed anti-H3N2 activity.
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