Histology Of The Organogenesis In Orange-Spotted Grouper (Epinephelus coioides) During Larvae Stage

• Main Authors: Yang Hui-Jun, 楊惠鈞
• Other Authors: Chang Poh-Shing
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/87686231251552867802

碩士 === 國立高雄海洋科技大學 === 水產養殖研究所 === 100 === Epinephelus coioides is the most common aquaculture species of high valuable. By high mortality due to food, metamorphosis chang and the other factors during larval rearing. Thus, a detailed understanding of the histology development of larval. In this study, histological method were observe the hatching to juvenile of organ and tissue development.The results showed at hatching larvae were mainly composed of basophilic undifferentiated tissue. The mouth and anus were not formed, the digestive tract was a straight tube bwteen dorsally and yolk sac with simple cuboidal epithelium. Appearance of Primordial maxilar, mandibular and fourth gill arches with cartilaginous frameworks at 2 days after hatching(DAH) . During this stage, the retina was differentiated into: photoreceptor layer, outer nuclear layer, outer plexiform layer, inner nuclear layer, inner plexiform layer, ganglion cell layer. On 3 DAH, mouth and anus were formed. The digestive tract was differentiated into: pharynx, esophagus, stomach, intestine and appears liver, pancreas, gall bladder. The first two parts and gall bladder was simple epithelium, that stomach was simple cuboidal epithelium, finally intestine was simple columnar epithelium, and found villis and microvillus in anterior intestine. A few goblet cells were found in the esophagus. Pancreas was basophilic cells containing some acidophilic secretions. Hepatocytes was polyhedral cell. To began formed renal tubule of body kidney. villis folds by a simple columnar epithelium and found a few goblet cells in the stomach. Many lipid vacuoles was in the anterior intestine epithelium, and acidophilic vacuoles was in the posterior intestine. Indicated presence of pinocytotic absorption and intracellular protein digestion, and lipid absorption. Heart differentiated into: atrium, ventricle, sinus venous, bulbus arteriosus. The ventricle consisted of cardiac muscle fiber. This stage show renal tubule and few hematopoiesis tissue in head kidney. The spleen also observed hematopoiesis tissue and red blood cell. Swimbladder was present and were visible gas gland. By 26 DAH telencephalon, mesencephalon, metencephalon, diencephalon, myelencephalon were evident, and could be observed nerve cell and nerve fibers. Could be observed pillar cells, rodlet cells, mucous cells and increased number of filaments. The formation of the trabecula to support thymocyte in thymus. Appearance the gastric glands and pyloric caeca on 36 DAH. The anterior intestine lumen villis full with scattered goblet cells, and acidophilic vacuoles has disappeared in the posterior intestine, that indicates the protein digestion mechanism has changed. By this time, the skin was stratified squamous epithelium. Appearance the scale and scale pocket of skin on 39 DAH. Finally the larvae were complete developed.