INSIGHT INTO AN ISSUE OF tRNA GENE COPIES AND CODON CAPTURE BY A GENOMIC VIEW OF Burkholderia pseudomallei

• Main Authors: Hsiu-Chuan Wang, 王秀娟
• Other Authors: Chen Ya-Lei
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/49912879273747044444

碩士 === 國立高雄師範大學 === 生物科技系 === 99 === The major function of tRNA was “Transfer” as protein combination. In mRNA-ribosome complex, the anticodon of tRNA was at 34-36 (3-2-1) base, and was the opposite complementary identification in mRNA code (1-2-3). The relation between codon and anticodon was coevolution, so the number of tRNA gene was impacted by wobble phenomena and charged-tRNA induced pre-translational modification. However, till now we didn’t have any research to verify this relation via genomic bio-information. Burkholderia pseudomallei had the most TRS serials in the genomes of prokaryotes, and it shown the multi-modification in gene evolution and could illustrate the relationship between the codon usage and the number of tRNA gene. In this research, we analyzed the gene of B. pseudomallei with tRNAScan-SE and define 59 functional tRNA gene. We excluded the wobble effect of A34, G34, U34 and I34-containing tRNAs and the interference of tRNAMetf gene, tRNASec isoaccepator gene, Q-containing tRNA in this research. Moreover, the physically codon number caught by tRNA was 22, and 56 tRNA gene, and established the relationship between codon usage and tRNA gene copies (r2 > 0.87). Besides, we also analyzed the relationship among Km value, Kcat value, Kcat/ Km of tRNA synthetase and tRNA gene copies, and this result demonstrates they didn’t have any relationship (r2< 0.01). This research pointed out that the positive relationship between the frequency of B. pseudomallei codon usage and tRNA gene copies, and excluded the effect to tRNA synthetase catalysis caught tRNA gene.