The Effect of SDF-1 Released from Chitosan/ Tripolyphosphate /Fucoidan Nanoparticles on Bone Marrow Mesenchymal Stem Cells

• Main Author: 劉庭儒
• Other Authors: Yi -Cheng Huang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/92629313993832857612

碩士 === 國立臺灣海洋大學 === 食品科學系 === 99 === Chemical factors and stem cells are important elements in tissue engineering. When tissue is damaged, specific chemokines are secreted to attract stem cells homing for tissue repair. Stromal cell derived factor-1 (SDF-1), massively released in injured tissue, affect the behaviors of stem cells and the effect of tissue repair. However, two drawbacks, short half-life and easily degraded by enzymes, limit the application of SDF-1. The aim of this study is to release SDF-1 from chitosan/tripolyphosphate/fucoidan (C/T/F) nanoparticles and evaluate its effect on stem cells. The experimental results show that the C/T/F nanoparticle is about 400 nm, and encapsulated SDF-1 effectively. The nanoparticles protected SDF-1 from deactivation by temperature and enzymes and controlled released SDF-1 up to 7 days. Moreover, fucoidan significantly affected the bioactivity and release amount of SDF-1. By in vitro cell experiments, the SDF-1 released from C/T/F nanoparticles improved cell proliferation, and attracted long-term Phosphoinositide-3-OH kinase(PI3K) expression and stem cell migration. In brief, the C/T/P nanoparticle shows a great potential in tissue engineering applications.