The Oncogenic Role of MALL in NSCLC

• Main Authors: Yen Chun-Lai, 賴彥君
• Other Authors: Sung-Liang Yu
• Format: Others
• Language: en_US
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/96812431839619968759

碩士 === 國立臺灣大學 === 醫學檢驗暨生物技術學研究所 === 99 === Background: Previous study showed that MALL can interact with Caveolin-1, and Caveolin-1 could enhance the invasive capability of lung adenocarcinoma cells by inducing filopodia formation. Therefore, we investigated the clinical significance of MALL expression in non–small-cell lung cancer (NSCLC) patients and its role in cancer progression. Methods: We enforced the expression of MALL in the cells with lower endogenous MALL expression (CL1-0, NCI-H226 and Hop62) and silenced MALL in those cells with higher endogenous MALL expression (CL1-5 and A549). The cell anchorage-independent growth, proliferation, cell migration, invasion, and in vivo turmorigenesis were analyzed in these transfectants. The potential downstream genes of MALL were identified by oligonucleotide microarray and quantitative reverse transcription–polymerase chain reaction (RT-PCR). In clinical correlation, we measured MALL expression in 90 tumor tissue of NSCLC patients by RT-PCR. Correlation between MALL expression and overall survival was determined by the log-rank test and multivariable Cox proportional hazards regression analysis. All statistical tests were two-sided. Results: We found that MALL can significantly enhance cancer invasiveness by the Boyden chamber matrigel assay (2.12 fold, p<0.01). MALL also promoted cell migration by the transwell assay (1.53 fold, p<0.01) and wound healing assay. Moreover, MALL increased the colonogenesis of lung cancer cells by using an anchorage-independent colony formation assay (1.79 fold, p<0.05) but did not affect the rate of cell proliferation by MTT assay. These findings indicated that MALL can promote tumor metastasis and tumorigenesis. Conclusion: MALL showed an oncogenic characteristic in NSCLC, and MALL might be a potential oncogene which might provide new insights into the future treatment of lung cancer.