Development of a Surface Acoustic Wave Based Micro-Droplet Control System and its Application of Nucleic Acid Amplification

• Main Authors: Ya-Lun Ho, 何亞倫
• Other Authors: 吳政忠
• Format: Others
• Language: en_US
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/43732841823831743871

碩士 === 國立臺灣大學 === 應用力學研究所 === 99 === In this thesis, an automatic micro-droplet control system applied to amplification of nucleic acid is accomplished by the combination of a surface acoustic wave (SAW) device, micro-heaters, micro-sensors of temperature, and a PI controller. The SAW device constituted of slanted finger interdigital transducers (SFITs) is utilized to actuate the micro-droplet by the acoustic streaming and to detect the micro-droplet by the frequency responses of the SAWs. With the development of PI controller, the micro-droplet can be manipulated automatically. In order to reduce the driving power and manipulate the mineral oil which is necessary for the reaction of the nucleic acid amplification, a perfluoroalkylsilane (PFAS) and tetraethoxysilane (TEOS) hydrophobic film is utilized for surface modification. Furthermore, with the PFAS/TEOS hydrophobic film, the cross-contamination can be prevented, and the micro-droplet control system is reusable for different DNAs and reagents. Utilizing the developed micro-droplet control system, the amplification of nucleic acid can be successfully achieved. The consumption of the biomedical reagent and the production cost of devices can be reduced, and the efficiency of reaction can be improved. The developed micro-droplet control system is suitable for various types of lab-on-a-chip system in biomedical fields.