A Study of Application of Carbon Paste Electrode Modified with Cobalt Hexacyanoferrate to Hydrogen Peroxide Sensor

• Main Authors: Ting-Li Lin, 林庭立
• Other Authors: Hau Lin
• Format: Others
• Language: zh-TW
• Published: 100
• Online Access: http://ndltd.ncl.edu.tw/handle/97152425807077348650

碩士 === 南台科技大學 === 化學工程與材枓工程系 === 99 === In recent years, sometimes the hydrogen peroxide is used in the food industry for the purpose of preservation and bleaching. Therefore, it is an important research subject to develop a rapid and convenient hydrogen peroxide sensor. Also, in recent years, the diabetes has become one of the top ten causes of death for the people in Taiwan. Therefore, developing a glucose biosensor which can detect the glucose rapidly, accurately, and conveniently is also an important research subject. The experimental design is widely used in many fields, and it is an important tool for the improvement of industrial process. A study of factorial design involving three factors (operating potential, stirring rate and pH value of phosphate solution) and two levels was performed to analyze the effect of reaction parameters on the sensitivity and average responding current of detection of hydrogen peroxide for the carbon paste electrode modified with Cobalt Hexacyanoferrate. The results of analysis of variance showed that the main effects of operating potential (F=380.493, p value < 0.05), stirring rate (F=70.053, p value < 0.05), and pH of phosphate solution (F=98.491, p value < 0.05) were significant on the sensitivity. The results showed that the optimum operating conditions for this study were operating potential = -200 mV, stirring rate = 700 rpm and pH of phosphate solution = 7.4. At 30℃, -200 mV operating potential, 700 rpm stirring rate and in 0.05 M phosphate solution (pH = 7.4), when the carbon paste electrode was modified with Cobalt Hexacyanoferrate (Cobalt Hexacyanoferrate : carbon powders : carbon paste = 3:7:10 (weight ratio)). The detection limit was 0.01 mM H2O2, the linear range was 0.01 ~1.2 mM H2O2, R2 = 0.9989 and the sensitivity was 213.86 μA / cm2．mM H2O2.