Inhibition of Gynura bicolor alcohol extract on intercellular adhesion molecule-1 expression

• Main Authors: Yun-shan Huang, 黃芸珊
• Other Authors: 王進琦
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/21983348916952971539

碩士 === 大仁科技大學 === 食品科技研究所 === 99 === Gynura bicolor (DC.), a common vegetable consumed in Taiwan, is well known for its dark green and purple leaves. Our found that Gynura bicolor alcohol extracts (GBAE) contain quercetin, rutin, gallic acid, chlorophyll and β-carotene of five major ingredients. In this study, we want find out whether the GBAE the antioxidant, anti-atherosclerosis predecessor factor expression of intercellular adhesion molecule -1 (ICAM-1) production and high fat diet rat reduction of atherosclerosis. From in vitro experimental model, 2 mg/ml water extracts of GBAE not only have a 1,1-diphenyl-2-picryl- hydrazy (DPPH) radical scavenging capability, but also have a ferrous ion chelating ability. Furthermore, the TBARS levels were significantly decreased when NRK-52E cells were treated with 50, 100 or 500 μg/ml GBAE and co-treated with t-butylhydroperoxide (t-BHP) (P<0.05). The total glutathione levels were significantly increased when NRK-52E cells were treated with 50, 100 or 500 μg/ml GBAE and co-treated with t-BHP (P<0.05). Activation of endothelium and inflammatory cells on early atherosclerosis of initiation and progression are required. Intercellular adhesion molecule-1 (ICAM-1) is one of the major factors in adsorption of peripheral blood leukocytes on the site of inflammation on endothelial cells. We investigated that effects of GBAE and its five ingredients on ICAM-1 expression on human umbilical vein endothelial cells (EA. hy 926 cells ) stimulated with tumor necrosis factor (TNF-α) in this study. Results show that 10, 50 or 100 μg/mL GBAE or five components of 100 μg/mL GBAE were all not affecting cell viability of EA. hy 926 cells. In addition, 100 μg/mL GBAE significantly inhibited the TNF-α-induced increase ICAM-1 in EA. hy 926 cells, but not separate all five ingredients. When cells treated with combined three ingredients (quercetin, rutin, IV gallic acid, M3 group) or five ingredients (quercetin, rutin, gallic acid, chlorophyll and β-carotene, M5 group), respectively, both groups can inhibit the ICAM-1 express in this study. Furthermore, GBAE and its active ingredients were significantly inhibited the levels of phosphorylation –IκB and reduced NF-κB-DNA binding activation on EA. hy 926 cells induced by TNF-α. In the animal experimental shown, 4, 8 and 16 mg/kg BW GBAE groups were not affect the growth character in HFD rats for 12 weeks. In the hematological parameters results shown GBAE 16 mg/kg BW were significantly increased WBC and reduce MCV level (P<0.05). HCT and PLT reduce whit 8 mg/kg BW GBAE treated (P<0.05). From liver biopsy examination, the liver fat accumulations were significantly descreased on all of GBAE groups in HFD rat by H&E stain. In addition, the levels of ICAM-1 in thoracic aorta were significantly decreased when SD rats were treated with GBAE for 12weeks. In conclusion, GBAE and its active ingredients could inhibit the ICAM-1 expression on EA. hy926 cells and SD rats in this study. And, regulation of NF-κB pathway by GBAE and its active ingredients may involve in this physiological affect. Therefor, GBAE maybe play a potential role in atherosclerosis prevention.