| Summary: | 碩士 === 慈濟大學 === 生命科學系碩士班 === 99 === The ABCDE model predicted the interaction of five classes of MADS box genes in regulating flower development in various plant species. In past decades, many of the MADS-box genes have been extensively studied in different plant species. The major MIKC-type MADS-box protein is characterized by the presence of four domains. These domains with different function to regulate the downstream genes through binding to the CArG box within the promoter region involved in the floral transition and floral development in plants. The E-function genes play essential role in the organ identity specification and express in all floral whorls. In previous, AGAMOUS-LIKE 2 homologue gene (LFAGL2) was isolated from Formosa lily (Lilium formosanum) through the strategy combining RT-PCR and RACE. Base on the sequence homology and phylogenetic tree analysis show that the MADS-box gene, LFAGL2, is classified into the AGL2 family. LFAGL2 mRNA was detected in the developing floral bud at different stages, tepal, stamen, carpel and silique, but absent in vegetative leaf. To characterize the function of LFAGL2 is able to regulate flower transition and initiation, LFAGL2 driven by CaMV 35S promoter was transform into the wild-type Arabidopsis thaliana that cause flowering early than wild type and defect in petal and stamen development in these transgenic lines. Ectopic expression of LFAGL2 can partial rescued the ap1 flower defect in transgenic Arabidopsis ap1 mutant plants . These results support that LFAGL2 is putative E-function gene in Formosalily and ectopic express in transgenic Arabidopsis plants can regulate the process of floral transition and floral organ identity.
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