| Summary: | 碩士 === 亞洲大學 === 生物科技學系碩士班 === 99 === Part I. The metabolic syndrome is a major risk factor for cardiovascular diseases and also raised the mortality. Cardiac apoptosis was found in metabolic syndrome such as obesity, diabetes, and hypertension. Very limited information regarding the effects of the supplementation of longan flower extracts in a fructose-fed rat model with metabolic syndrome. Male Sprague-Dawley rats were divided into five groups fed with standard Purina chow (Control group, C), high fructose diet (HF), high fructose diet plus longan flower water extract 250 mg/kg per day by gavage (LFWE), high fructose diet plus longan flower ethyl acetate fraction 36.3 mg/kg per day by gavage (LFEA), high fructose diet plus longan flower water fraction 147.5 mg/kg per day by gavage (LFW). After 16 weeks, hearts were measured by H&E stain, TUNEL assay and western blotting. Protein levels of Fas-dependent and mitochondria dependent apoptotic pathway, such as Fas, FADD, Bak, Cytochrome c, activated caspase-9 and activated caspase-3 were increased in HF group compared with C group, whereas those were decreased in LFWE, LFEA and LFW groups compared with HF group; and survival related proteins (IGF1-receptor, p-Akt, p-Bad, Bcl-2, Bcl-xL) were decreased in HF group compared with C group, whereas those were increased in LFWE, LFEA and LFW groups compared with HF group. Longan flower treatment might suppress cardiac apoptotic pathways and elevated cardiac survival pathway in fructose-fed rat model. The findings suggest that longan flower may be one of the possible therapeutic agents for preventing cardiac apoptosis in metabolic syndrome.
Part II. Cardiac apoptosis and fibrosis was found in aging-related diseases. Very limited information regarding the effects of the supplementation of Diosgenin in a D-galactose-induced aging model. Male Wistar rats were divided into Control group (S0), aging group (D0), and D0 groups with 10mg/kg and 50mg/kg diosgenin spp.daily (DD10 and DD50). After 8 weeks, hearts were measured by H&E stain, TUNEL assay and western blotting. Protein levels of Fas-dependent and mitochondria dependent apoptotic pathway, were increased in D0 group compared with S0 group, whereas those were decreased in DD10 and DD50 groups compared with D0 group; and survival related proteins, were decreased in D0 group compared with S0 group, whereas those were increased in DD10 and DD50 groups compared with D0 group. Fibrosis related proteins, were increased in D0 group compared with S0 group, whereas those were decreased in DD10 and DD50 groups compared with D0 group. Diosgenin treatment might suppress cardiac apoptotic and fibrosis pathways and elevated cardiac survival pathway in D-galactose-induced aging model. The findings suggest that Diosgenin may be one of the possible therapeutic agents for preventing cardiac apoptosis in aging-related diseases.
|
|---|
