Investigating the effects on the preparation of monodisperse SBA-15 microsphere and its applications for protein drug delivery.

• Main Authors: Kuo, Hualiang, 郭華良
• Other Authors: Gu, Yesong
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/02490037939979753956

碩士 === 東海大學 === 化學工程與材料工程學系 === 99 === The morphology and dispersion of SBA-15 particles could influence the biocompatibility and the applications for drug delivery systems. Therefore, this study is to investigate the conditions to synthesize well-dispersed spherical SBA-15 for drug delivery system and apply it to immobilize protein. The components used for SBA-15 should be introduced, at the first the research has shown that the concentration of hydrochloric acid could influence ionic strength of the reaction solution, and influence both hydrophobic and hydrophilic chain of triblock copolymer P123. Besides, TMB would be easy to enter into the center of micelle, and then obtain a consistent pore morphology and narrow pore distribution. Furthermore, we found an appropriate concentration (2 M HCl) could increase zeta-potential of SBA-15 and well-dispersed spherical SBA-15 was obtained. However the higher hydrogen concentration (3 M HCl) caused the fast condensation polymerization, and resulted in serious aggergation. The ionic strength of solution can also be increased by adding various salts. Salts could lead the desolvation (dehydration) of the hydrophobic chain, and stabilize the micelle by enhancing the assembling of hydrophobic chain. Besides, it is also making the swelling-agent TMB into the center of micelle well. Adding swelling-agent TMB can change the micelle size, then to control the morphology and pore size distribution of SBA-15. At the same time, the difference of TMB to enter into micelle center is dependening on hydrochloric acid and salts concentrations. This study has also shown that the charge between the carrier and protein would have a great influence on protein adsorption by carrier. For example, when the carrier and lysozyme displaced opposite different charges on their surface, the amount of protein adsorbed will be 3 times higher than that for the carrier and BSA in which has same charge type. Because of the electronstatic force between carrier and protein, we can clearly observe drug slow-release phenomenon on lysozyme releasing test. Therefore, it proves the feasibility for SBA-15 as a carrier for macromolecular drugs (protein) delievery system.