Development, preparation, and application of FePt magnetic nanoparticles.

• Main Authors: Huang, Lin-Yu, 黃琳育
• Other Authors: Ho, Chih-Sung
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/67116601098904745379

碩士 === 東海大學 === 化學工程與材料工程學系 === 99 === In recent years, nanoparticles are applied in biomedical researches because of their magnetic, optical, and electrical properties. Nanoparticles are used for label, detection, and medical purposes. Magnetic nanoparticles of different shapes, sizes, and formations can be prepared by different chemical synthesis methods. In this study, the hydrophobic FePt nanoparticles are prepared by chemical reduction method. The ratio of surfactants to precursors as the parameter is used to control the size and the dispersion of nanoparticles. The nanoparticles transfer from hydrophobic to hydrophilic when the thiol group is used for surface modification. The properties of nanoparticles are studied by XRD, TEM, and VSM. The hydrophilic FePt magnetic nanoparticles are conjugated with HRP by EDC cross-linker. The absorption spectra of different concentrations of HRP are measured by the UV/visible spectrophotometer after HRP reacts with ABTS substrate. The intensity of spectrum varies with the concentration of HRP providing the availability for quantitative analysis.