| Summary: | 碩士 === 大同大學 === 生物工程學系(所) === 99 === The effect of cultural factors ( MS basal salt strength, NH4NO3 and sucrose) on proliferation and total flavonoids production in adventitious roots of Belamcanda chinensis were studied. The optimum culture conditions for biomass increase and total flavonoids production were full strength MS macrosalts , NH4NO3 at 7.19 mM and sucrose at 3 % . The best growth ratio of adventitious roots was 5.9±0.3 under optimum condition in flask culture. The growth ratio can reached to 4.0±0.6 and the accumulation of total flavonoids was 48.1±5.4 eq. iridin mg/g DW and total isoflavonoids was 38.3±0.3 mg/g DW in adventitious roots under the same culture conditions in 1L bubble column bioreactor. Adventitious roots treated with antimitotic agents at different concentration (0.5 , 1 and 3 mg/l) and time (1 , 2 and 3 weeks) and five adventitious root lines (1WC0.5, 2WO0.5 , 2WC1 , 3WC1 and 3WT0.5) were selected (growth ratio＞20). Among five lines, the 3WC1 line showed the best growth rate was 36.3 cultured in NH4NO3 at 7.19 mM of modified MS medium. The highest total flavonoids (94.1±1.7 eq. iridin mg/g DW) and isorhamnetin (36.9±1.7 mg/g DW) production were expressed in 3WT0.5 line and the maximum total isoflavonoids production of 63.3±7.8 mg/g DW was occurred in 1WC0.5 line cultured in MS medium without NH4NO3.
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