Surface Acoustic Wave and Molecularly Imprinted Polymer Biosensor for Detection of Creatine Kinase-MM

• Main Authors: Chih-Ying Lin, 林志穎
• Other Authors: Chung-Yih Wang
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/39739913069253067225

碩士 === 大同大學 === 生物工程學系(所) === 99 === This study used Lithium Tantalite produced surface acoustic wave devices using interdigital transducers with a width of 5 μm and a thickness of 75 nm, and a theoretical center frequency of 108 M Hz. The surface acoustic wave devices were coated with a 200 nm thick silicon dioxide layer to protect the interdigital transducers. We used a molecular imprinting polymer aggregation process on the surface of the acoustic wave devices to form imprints of the Creatine Kinase. Then we selected the functional monomer Methacrylic acid (MAA), and a cross-linking agent Poly (ethylene glycol) dimethacrylate (PEG400DMA, Mn = 550) with MAA and PEG400DMA with a concentration of 5:95 (v/v), and together with photoinitiators and 1 mg of Dimethoxy-2-phenylacetophenon, and then using UV polymerization for 10 minutes, we found that the creatine kinase and the molecular imprinted polymers bond between the formation of protein identification holes of the molecular imprinted polymers. Taking advantage of this feature of re-bonding creatine kinase molecules, a network analyzer can be used to detect changes in the insertion loss of surface acoustic wave devices. We polymerized the concentration 0.025 mg / ml Creatine Kinase at the SAW–MIP sensor test area and using the insertion loss from the previous test result as a marker. When the creatine kinase was removed from the molecularly imprinted polymers, the insertion loss increased to 0.8035 dB; which then adsorbed 2 μg / mL creatine kinase and the insertion loss increased by 0.1511 dB; which then adsorbed 1 μg / mL creatine kinase, and the insertion loss increased by 0.3406 dB; which then adsorbed 0.5 μg / mL of creatine kinase, and the insertion loss increased by 0.3602 dB; which then adsorbed 0.25 μg / mL of creatine kinase, and the insertion loss increased by 0.4818 dB. If the non-molecularly imprinted polymers were washed before and after then the insertion loss change was insubstantial .