| Summary: | 碩士 === 元智大學 === 化學工程與材料科學學系 === 99 === The polyhydroxyalkanoates(PHA) have been recognized as good substitutes for the non-biodegradable petro-chemically produced polymers. It can be generated from microbial and regarded as a potential material in several of industrial application. In this study, the crude glycerol (CG) as the carbon source was supplied to Ralstonia eutropha H16 for PHAs accumulation. The economic effect of using glycerol compared to other carbon sources was analyzed in respect of productivity and material cost. The composition of crude glycerol was provided by biodiesel company in Taiwan. It composited 80 %of glycerol, 11 %water, 9 %metal ions and un-puritys.
The optimization production was carried out to study the influences of medium conditions supply, temperature and inoculums ratio. The optimization temperature and inoculums ratio for PHAs formation is observed at 30℃ and 10 % (v/v), respectively. The dry cell weight(DCW) can achieve average 4 g L-1 and PHB content was found in the range of 25%~ 45 % (w/w)。
This study was successful used define medium add CG as carbon source culturing Ralstonia eutropha H16. A 2 L fermentor for the batch fermentor of the key factors, the experimental results showed that dissolved oxygen must be greater than 50%, pH does not control, aeration of 1 vvm, the slot for the fermentation conditions optimization.
The fermentor scale up to 5 L and 50 L, in the 5L fermentor for 72 hours, PHAs content obtained was 75.1 % (w/w), cell dry weight increased to 38.41 g L-1 and glycerol utilization up to 85.2 %. Each gram of glycerol can produce 0.67 g of PHB, and PHB yield up to 0.40 g hr-1 L-1, reduce the cost of raw materials to 6.14 USD kg-1 PHB.
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