| Summary: | 碩士 === 元智大學 === 生物科技與工程研究所 === 99 === Sweet potato, Ipomoea batatas (L.) Lam., is the 7th most important crop in the world. The tuberous roots and leaves for certain varieties of sweet potato are good nutrient source for human beings. Because of low requirement for growth environments, sweet potato becomes one of prospective host for plant molecular farming. We successfully developed gene transformation system in sweet potato. Therefore, we offer a new approach to increase the economic value of sweet potato in Taiwan through molecular farming proposed here. We apply this gene transformation system to produce the recombinant mouse Granulocyte-macrophage colony stimulating factor (mGM-CSF)proteins in transgenic sweet potato, the mouse GM-CSF can affect the mouse white blood
increase and growth, In rent year, the mGM-CSF use in mouse modern rescute, including therapy cancer and tumor, It is so expensive and necessary much more to research and rescute. It，s 5 ug sold NT 8000 dollars now. by either constitutive strong ubiquitin promoter or root overexpression promoters. (SPOA1 or PGI1) transformation to sweet potato, hope to overexpression recombination protein mGM-CSF. In our research, we establish five sweet potato transgenic cell lines pUBI::mGMCSF, use the genomic DNA PCR、RT-PCR、GUS reporter gene check and western blot analysis to check the recombination protein overexpression in sweet potato also succeed regeneration two lines to a sweet potato transgenic lines (CSF-4 and CSF-25),analysis had two regeneration transgenic sweet potato lines, the two lines still have the mGMCSF overxpression.
In the other hand, we succeed construction the SPOA1::mGMCSF，and try to use the sweet potato transformation system, and try to obtain the transgenic line.In other hand,we use the PGI promoter from the Arabidopsis, we hope use the transformation of sweet potato system, try to understand where the PGI1 driven GUS can overexpression in sweet potato. In future, we hope to overexpression in sweet potato tuberous root. To use the advantage of tuberous root production of mGM-CSF protein.
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