| Summary: | 碩士 === 國立中正大學 === 分子生物研究所 === 100 === Sex-determining region Y (SRY)-box protein 2 (SOX2), a member of the HMG transcription factor family, plays a critical role in maintenance of stem cells. Expression of SOX2 in the lung epithelium is sufficient for development of adenocarcinoma with the molecular signature of squamous carcinoma, implicating SOX2 as an oncogene. However, SOX2 is frequently down-regulated in gastric cancers in which SOX2 functions to inhibit cell growth through cell-cycle arrest and induction of apoptosis. We have discovered that SOX2 is up-regulated in the urothelial carcinoma cell lines BFTC905 and that SOX2 exhibits RNA binding activity. However, the function of SOX2 in development and progression of uproot helical carcinoma is not clear, and the molecular mechanisms SOX2 participates through its RNA binding activity is not yet defined. Thus, the goal of my thesis is to investigate the molecular mechanisms underlying the development of urothelial carcinoma.
To determine the function of SOX2 expression in BFTC905, we established a BFTC905 cell lines with SOX2 expression knocked down by shRNA. In these cell lines, the RNA level and the protein level of S100A14 is reduced, and, coupled with our previous finding that SOX2 binds to the S100A14 mRNA, the result suggests that SOX2 plays an important role in regulating translation of specific mRNAs. On the other hand, we also determine whether the growth rate and the migration ability of BFTC905 is altered by suppression of SOX2 expression. The preliminary results that suppression of SOX2 appears to increase the growth and the migration of BFTC905. However, additional experiments taking different techniques and approaches are required to confirm these finding. In the near, future I will re-confirm my preliminary findings and investigate the role of SOX2 in post-transcriptional mRNA regulation.
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