Automatic Cell Counting For Hemocytometers Through Image Processing

• Main Authors: Chen, Yuwei, 陳裕瑋
• Other Authors: Chiang, Peiju
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/38107525703686033550

碩士 === 國立中正大學 === 機械工程學系暨研究所 === 100 === Cell counting is usually done manually for in vitro cell culture using a hemocytometer. It is important that cell counts are accurate, consistent, and fast, particularly for quantitative measurements of cellular responses. Although some automated cell counting devices were developed, the prices are not favorable. In contrast, hemocytometer is inexpensive and commonly used in lab, but it is laborious and subject to user bias and misuse, which results in inaccurate counts. To mitigate the tedium associated with manual counting and reduce the user bias, we proposed to develop an automatic cell counting method for Hemocymeters by image processing.