Investigation of the role of GPR56 in human NK cell biology

• Main Authors: Mei Hui Huang, 黃美惠
• Other Authors: H. H. Lin
• Format: Others
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/33157544982743383327

碩士 === 長庚大學 === 生物醫學研究所 === 100 === Natural killer (NK) cells play an important role in innate immunity. NK cells possess a natural cytotoxic potential against virus-infected cells and tumor cells without priming. NK cells secret cytokines to coordinate innate and adaptive immunity by a variety of germ-line encoded receptors that can be classified into activating, inhibitory, adhesion, cytokine and chemokine receptors. Recently, a specific member of the G protein-coupled receptors, GPR56 has been found to be abundantly expressed in NK cells. However, its function has remained mysterious. GPR56 is involved in cell to cell, cell and matrix interaction in other cell types. GPR56 is able to associate with CD81. We used a stable GPR56 over-expressing NK92 cell line and specific antibody stimuli on fresh peripheral blood NK cells to investigate the biological functions of GPR56 in NK cells. Our data suggests that GPR56 is able to regulate the motility of NK cells, affect cytokine secretion and cytotoxicity when recognizing target cells. Indeed, GPR56 might interact with CD81 to regulate the biological functions of NK cells.