Development of graphene sensing membranes using for ISFET biosensor applications
碩士 === 長庚大學 === 電子工程學系 === 100 === Alanine aminotransferase (ALT) has been regarded as one of the most sensitive indicators of hepatocellular damage. While ALT is widely used in the practice of medicine, few attempts have been made to develop biosensors applicable to the electrolyte-gated graphene f...
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| Format: | Others |
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2012
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| Online Access: | http://ndltd.ncl.edu.tw/handle/68797071465779791726 |
| Summary: | 碩士 === 長庚大學 === 電子工程學系 === 100 === Alanine aminotransferase (ALT) has been regarded as one of the most sensitive indicators of hepatocellular damage. While ALT is widely used in the practice of medicine, few attempts have been made to develop biosensors applicable to the electrolyte-gated graphene field-effect transistors (GFETs). The single-layer graphene was used as a channel. First, GFETs immersed in an electrolyte showed their conductances exhibited a direct linear increase with electrolyte pH, indicating their potential for use in pH sensor applications. Then, we use the entrapment method to immobilize alanine transaminase enzyme on the surface by the reaction with Alginate and CaCl2 solution and showed that the conductance of GFETs increased with exposure to a ALT at 10~100 U/L. The GFETs thus acted as highly sensitive electrical sensors for detecting pH and biomolecule concentrations.
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