| Summary: | 碩士 === 長庚大學 === 物理治療學系 === 100 === Background and Purpose: Bone marrow-derived, circulating progenitor cells (CPCs) is contributing to the maintenance of endothelial function by mechanisms that endothelial repair to postnatal angiogenesis and vasculogenesis. Although exercise has been suggested that can upregulate CPCs number in blood, the effect of different mode of exercise on CPCs subsets remains unclear. High intensity aerobic interval training (AIT) has been shown to have a more profound influence on cardiovascular function and aerobic capacity than isocaloric moderate intensity continue training (MCT). The aim of this study was to investigate how AIT influences the mobilization of CPCs subsets into peripheral blood compartment. A second objective was to compare MCT with AIT that the adaptation of ischemic and hypoxic hypoxia changing in cell number and function. Methods: Thirty-two sedentary healthy men were participating in the study, which were randomly distributed to AIT exercise group (reciprocal 40%, 80%VO2max on a bicycle ergometer for 30min per day, 5 days per week, total 25 times) or MCT exercise group (60%VO2max). Acute severity hypoxia exercise testing was performed at pre and post chronic exercise training (60% VO2 max exercise intensity for 40 min under 12%O2 in air). At rest and immediately after severity hypoxia exercise, total of stem cells (SCs, CD34+ cells), circulating angiogenesis cells (CACs, CD34+/KDR+ cells), hematopoietic stem cells (HSCs, CD34+/KDR+/CD117+ cells), endothelial progenitor cells (EPCs, CD34+/KDR+/CD133+ cells), circulation endothelial cells (CECs, CD34+/KDR+/CD31+ cells), and the apoptosic cells number of CACs (CD34+/KDR+/phosphotidylserine exposed cells) were measured by three-color flow cytometry. Subsequently, we measured the ability of CPCs and subtypes cell proliferation by cell culture. Result：Exercise training improved cardiopulmonary fitness in AIT and MCT group. In blood analysis, acute hypoxia exercise testing could improve CACs and EPCs in first time. After five weeks training, AIT group increased the number of SCs, CACs and EPCs in peripheral blood compartment and MCT group significantly increased the number of CACs in the blood. Conclusion：Aerobic interval training markedly induced SCs differentiation to the functional CPCs into peripheral blood. Clinic Application and Future Research ：This study clarify the relationships between AIT and MCT modulated redistribution of CPCs. In future, require more research relevant to CPCs differentiation and proliferation capacity and improve angiogenesis ability in vivo by physical therapy method. We expect to provide a safe and effective strategy of exercise therapy for patients with endothelial dysfunction.
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