| Summary: | 碩士 === 中國醫藥大學 === 中國藥學暨中藥資源學系碩士班 === 100 === Cerebral and cardiovascular diseases are mainly caused by the pathogenesis of arterial vascular smooth muscle. Paeonia suffruticosa Andr. (abbreviated as PSex)is a well-known traditional Chinese herbal medicine. Ancient Chinese medicine literatures have showed that various effects of this herb on human sickness such as reducing heat, promoting blood flow and eliminating stasis. Paeonol (2′-hydroxy-4′-methoxyacetophenone) is one of the main components extracted from PSex. Paeonol was reported to have multi-biological activites such as anti-inflammatory, anti-allergic, anti-tumor, anti-angiogenic, and anti-oxidative activities. In the present study, the effects and action mechanism of PSex and paeonol on vascular smooth muscle cells proliferation and migration were evaluated.
Results from our study revealed that PSex reduced hyperplasia in the mouse carotid ligation model. Low-dose (0.1 g/kg) or high-dose (0.2 g/kg) of PSex decreased the level of intima/media (I/M) ratio in 24 % and 37 % compared with the control group, respectively. PSex reduced PCNA positive cells in a dose-dependent manner and the inhibition rate was 35 % (0.1 g/kg) and 62 % (0.2 g/kg) compared with control. In wound healing assay and transwell migration assay. The results showed that PSex and paeonol extracts significantly inhibited platelet-derived growth factor (PDGF) induced A7r5 cells migration and proliferation. PSex and paeonol decreased Ras, MEK, p-MEK, p-ERK1/2 and MMP-2 and MMP-9protein level in western blotting analysis.
In conclusion, results from this study revealed that PSex inhibited
10
carotid-ligation induced intimal hyperplasia; PSex and paeonol both inhibited PDGF –induced A7r5 cell proliferation and migration which might be via the inactivation of MMP2, MMP9, and MEK/ERK pathway
|
|---|
