Studies on the Chemical Constituents from the Root of Echinope grijisii Hance
碩士 === 中國醫藥大學 === 藥物化學研究所碩士班 === 100 === Echinops grijisii Hance (Echinops) is a folk medicine used in Taiwain to treat inflammation and detoxification.Our preliminary bioassay test in dicated that the methanol extract of radix of this plant showed a dose-dependent effect to NO inhibition.We continu...
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ndltd-TW-100CMCH55490112015-10-13T21:32:33Z http://ndltd.ncl.edu.tw/handle/62098946176491177176 Studies on the Chemical Constituents from the Root of Echinope grijisii Hance 山防風根部化學成分之研究 Fang-Pin Chang 張芳賓 碩士 中國醫藥大學 藥物化學研究所碩士班 100 Echinops grijisii Hance (Echinops) is a folk medicine used in Taiwain to treat inflammation and detoxification.Our preliminary bioassay test in dicated that the methanol extract of radix of this plant showed a dose-dependent effect to NO inhibition.We continue in the study on chemical constituents of this plant. These dried stems of Echinops grijisii Hance were extracted with methanol.The extracts were further separated by chromathography.Tirty six compounds have been isolated and purified. Those include : Six aromatics: Methyl caffeate (AR 1), 4,5-dimethoxybenzene-1,2-dicarbaldehyde (AR 2**), Methyl paraben (AR 3), 3-(4’-Hydroxyphenyl)-(E)-propenoic acid methyl ester (AR 4), Methyl veratrate (AR 5), and Vanillin (AR 6) Five anthraquinone: Chrysoobtusin (AQ 1), Obtusin (AQ 2) , Aurantio-obtusin (AQ 3) , 1-desmethylobtusin (AQ 4) , and Obtusifoline (AQ 5) Eigteen thiophens: 2,2''-bithiophene -5''-carboxylic-acid (TH 1**), 2-(3,4-dihydroxybut-1-ynyl)-5-(penta-1,3-diynyl) –thiophene (TH 2), α-Terthienyl (TH 3) , 5’-(3,4-Dihydroxybut-1-ynyl)-2,2’-bithiophene (TH 4) , 5-acetyl-2,2''-bithiophene (TH 5) , 5-formyl-2,2''-bithiophene(TH 6**) , methyl-5,2''-bithiophene-2-carboxylate (TH 7**) , 5''-(But-3-en-1-ynyl)-2,2''-bithiophene (TH 8) , 5''-(4-hydroxy-1-butynyl)-2,2''-bithiophene (TH 9) , Cardopatine (TH 10) , Isocardopatine (TH 11) , 5''-(3-Hydroxy-4-isovaleroyloxybut-1-ynyl)-2,2’-bithiophene (TH 12) , 5''-(3-Hydroxy-4-isovaleroyloxybut-1-ynyl)-2,2''-bithiophene (TH 13) , 5-(4-hydroxy-1-butynyl)-2,2''-bithiophene (TH 14) , 5’-(4-acetoxy -1-butynl)bithiophene (TH 15) , 5’-(4- hydroxy -3-methoxy-1-butyny)bithiophene (TH 16*) , 2-(penta-1, 3-diynyl)-5-(4-hydroxybut-1-ynyl)-thiophene(TH 17), 5’-(3- Hydroxy -4-acetoxybutyl-1-butyny)2,2’bithiophene(TH 18) Four steroids: β-Sitosterol (ST 1), Stigmasterol (ST 2), β-Sitosterol-O-D-Glucoside (ST 3), β-Stigmasterol-O-D-Glucoside (ST 4) one lignan: Pinoresinol (LI 1) Two Sesquiterpenes: Dihydrosantamarine ( SQ 1), Santamarine ( SQ 2) According to bioassay result , compound AQ 1(Chryso obtusin ) showed the best cell viability to RAW 264.7 cell;compound SQ 2 (Santamarine ) showed the most inhibitory activity against LPS-induced NO production with IC50 value of 4.59 ± 0.17 μg/mL. *New compound ** First isolation from natural source Jin Bin Wu 吳金濱 2012 學位論文 ; thesis 162 zh-TW |
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碩士 === 中國醫藥大學 === 藥物化學研究所碩士班 === 100 === Echinops grijisii Hance (Echinops) is a folk medicine used in Taiwain to treat inflammation and detoxification.Our preliminary bioassay test in dicated that the methanol extract of radix of this plant showed a dose-dependent effect to NO inhibition.We continue in the study on chemical constituents of this plant.
These dried stems of Echinops grijisii Hance were extracted with methanol.The extracts were further separated by chromathography.Tirty six compounds have been isolated and purified. Those include :
Six aromatics:
Methyl caffeate (AR 1), 4,5-dimethoxybenzene-1,2-dicarbaldehyde (AR 2**), Methyl paraben (AR 3), 3-(4’-Hydroxyphenyl)-(E)-propenoic acid methyl ester (AR 4), Methyl veratrate (AR 5), and Vanillin (AR 6)
Five anthraquinone:
Chrysoobtusin (AQ 1), Obtusin (AQ 2) , Aurantio-obtusin (AQ 3) , 1-desmethylobtusin (AQ 4) , and Obtusifoline (AQ 5)
Eigteen thiophens:
2,2''-bithiophene -5''-carboxylic-acid (TH 1**), 2-(3,4-dihydroxybut-1-ynyl)-5-(penta-1,3-diynyl) –thiophene (TH 2), α-Terthienyl (TH 3) , 5’-(3,4-Dihydroxybut-1-ynyl)-2,2’-bithiophene
(TH 4) , 5-acetyl-2,2''-bithiophene (TH 5) , 5-formyl-2,2''-bithiophene(TH 6**) , methyl-5,2''-bithiophene-2-carboxylate (TH 7**) , 5''-(But-3-en-1-ynyl)-2,2''-bithiophene (TH 8) , 5''-(4-hydroxy-1-butynyl)-2,2''-bithiophene (TH 9) , Cardopatine (TH 10) , Isocardopatine (TH 11) , 5''-(3-Hydroxy-4-isovaleroyloxybut-1-ynyl)-2,2’-bithiophene (TH 12) , 5''-(3-Hydroxy-4-isovaleroyloxybut-1-ynyl)-2,2''-bithiophene (TH 13) , 5-(4-hydroxy-1-butynyl)-2,2''-bithiophene (TH 14) , 5’-(4-acetoxy -1-butynl)bithiophene (TH 15) , 5’-(4- hydroxy -3-methoxy-1-butyny)bithiophene (TH 16*) , 2-(penta-1, 3-diynyl)-5-(4-hydroxybut-1-ynyl)-thiophene(TH 17), 5’-(3- Hydroxy -4-acetoxybutyl-1-butyny)2,2’bithiophene(TH 18)
Four steroids:
β-Sitosterol (ST 1), Stigmasterol (ST 2), β-Sitosterol-O-D-Glucoside (ST 3), β-Stigmasterol-O-D-Glucoside (ST 4)
one lignan:
Pinoresinol (LI 1)
Two Sesquiterpenes:
Dihydrosantamarine ( SQ 1), Santamarine ( SQ 2)
According to bioassay result , compound AQ 1(Chryso obtusin ) showed the best cell viability to RAW 264.7 cell;compound SQ 2 (Santamarine ) showed the most inhibitory activity against LPS-induced NO production with IC50 value of 4.59 ± 0.17 μg/mL.
*New compound
** First isolation from natural source
|
author2 |
Jin Bin Wu |
author_facet |
Jin Bin Wu Fang-Pin Chang 張芳賓 |
author |
Fang-Pin Chang 張芳賓 |
spellingShingle |
Fang-Pin Chang 張芳賓 Studies on the Chemical Constituents from the Root of Echinope grijisii Hance |
author_sort |
Fang-Pin Chang |
title |
Studies on the Chemical Constituents from the Root of Echinope grijisii Hance |
title_short |
Studies on the Chemical Constituents from the Root of Echinope grijisii Hance |
title_full |
Studies on the Chemical Constituents from the Root of Echinope grijisii Hance |
title_fullStr |
Studies on the Chemical Constituents from the Root of Echinope grijisii Hance |
title_full_unstemmed |
Studies on the Chemical Constituents from the Root of Echinope grijisii Hance |
title_sort |
studies on the chemical constituents from the root of echinope grijisii hance |
publishDate |
2012 |
url |
http://ndltd.ncl.edu.tw/handle/62098946176491177176 |
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