| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 100 === Staphylococcus aureus is Gram-positive spherical bacteria. It causes a variety of suppurative (pus-forming)infections and toxicity in human. It causes superficial skin lesions such as boils, styes and furunculosis; more seriousinfections such as pneumonia, mastitis, phlebitis, meningitis, and urin ary tractinfections; and deep- seated infections, such as osteomyelitis and endocarditis. S.aureus is a major cause of hospital acquired (nosocomial) infection of surgicalwounds and infections associated with indwelling medical devices. S.aureus causes food poisoning by releasing endotoxins into food, and toxic shoch syndrome by releasing superantigens into the blood stream.
Conventional methods for determining S. aureus were colony hybridization,preculture and selective medium and biotype test etc. However, these methods needed long procedure, specific machine for determination. The objective of this study was to develop a self-assembled immunochromatographic strip for rapid determination of S. aureus using rabbit anti-Protein A polyclonal antibodies(pAbs). Protein A is a surface protein of S. aureus. This self-assembled immunochromatographic strip was convenient, rapid, low price and no machine needed in clinical diagnosis. The colloidal gold nanoparticles were made from the chloroauric acid (HAuCl4) and anti-Protein A were labeled with 25 nm of colloidal gold.
This immunochromatographic strip prepared could determine for 0.01-100 μg/mL Protein A and no cross-reaction. Moreover, the detection limit IVof this strip for S. aureus samples was about 196cells/mL, and the detection time was 10-20 min.
This study was to develop a self-assembled immunochromatographic strip for determination of S. aureus in environments. The samples were collected from automatic teller machines (ATMs), tea drinks, computer keyboards,hospital beds, cabinets of hospital, used powder puffs, hand staircases with a handrail and water fountains. The total numbers of samples were 36. In which,the majority of positive reactions in all samples were obtained from the determination of ATMs.
The advantages of the self-assembled immunochromatographic strip were high specificity, high sensitivity, no pretreatment, low sample requirement, easy operation, rapid determination, low price, no cross-reaction, long-term preservation, no machine needed etc.
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