Investigation of Neosartorya fischeri NRRL 181 gene cloning and secondary metabolites prodution

• Main Authors: Gen-Lin Shu, 許敬琳
• Other Authors: Jui-Hsiang Hung
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/g49s46

碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 100 === Fungal secondary metabolites are an important source of bioactive compounds for agricultural and medical. A survey of the literature on 1,500 secondary metabolites isolated and characterized between 1993 and 2001 revealed that over half of these compounds have antibacterial, antifungal, or antitumor activity. In the past data, Neosartorya fischeri contains 16 sets of PKS and 18 sets of NRPS gene clusters to synthesize prediction of specific secondary metabolites. The present study, Neosartorya fischeri secondary metabolites related literature is very scarce, Therefore, from Neosartorya fischeri genome prediction has been shown, there are large number of secondary metabolites has not been found. This is probably because many secondary metabolite gene clusters are not expressed under normal laboratory culture conditions. In this study, the use of fusion PCR completed which NFIA_043670 fusion gene, and NFIA_043680, NFIA_043690 PyroA/WA3’cloning. In the experiment, with the change to a different carbon source to investigate the performance of the secondary metabolites, the use of glucose as a carbon source for the control group, monosaccharides of xylose, fructose and the disaccharide of sucrose and lactose as the experimental group, and the high performance liquid chromatography detection found that the area under the curve best performance, glucose carbon source is xylose carbon source, followed by fructose, sucrose, lactose manifested area under the curve worst, this experiment also added ammonium chloride as nitrogen sources found poor effect of secondary metabolites produced in this experiment in turn to separate out from Neosartorya fischerisecondary metabolites as antibacterial test, does not have any effect found in Pseudomonas aeruginosa , Salmonella and E. coli.