The effects of conjugated linoleic acid on the antiobesity and antioxidant systems in mice fed with high-fat diet

• Main Authors: Chao-pin Kung, 孔昭蘋
• Other Authors: Huey-mei Shaw
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/333gc4

碩士 === 嘉南藥理科技大學 === 保健營養系 === 100 === Conjugated linoleic acid (CLA) refers to a group of positional and geometric isomers of the omega-6 essential fatty acid, linoleic acid. The reduction effect of CLA on the adipose tissue and body weight in mice has been noticed and widely studied. In our previous study, CLA also increased vitamin E status in mice fed with low-fat diet. The aim of this study was to investigate the effects of conjugated linoleic acid on the antiobesity and antioxidants systems in mice fed with high-fat diet. Twenty-four seven-week-old male C57BL/6J mice were assigned to three groups: LF group (containing 5% soybean oil), HF group (containing 3% soybean oil of +17% lard), and CLA group (containing 2% soybean oil +1% CLA +17% lard) were fed with experimental diets for 8 wk. The results showed that α-tocopherol levels of plasma and liver were significantly highest in CLA group. The TBARS concentration of kidney in CLA group was significantly lower than that in LF group (P < 0.05). The TBARS concentration of lung in CLA group was significantly lower than that in HF group (P < 0.05). The levels of urine 8-isoprostane significantly increased in CLA fed mice. CLA group showed a reduction of COX-2 protein expression in liver. In addition, the activities of CAT, SOD, GPx of liver in CLA group showed significantly lower than that in the other two groups (P <0.05). The concentration of plasma adiponectin was significantly lower in CLA group than that in the other two groups. In CLA group, the cholesterol levels in plasma and the triglyceride levels in liver both showed significantly higher than the other groups. In conclusion, CLA could reduced the retroperitoneal fat and epididymal fat mice fed with high-fat diet. CLA increased the α-tocopherol content in plasma and liver and decreased plasma adiponectin levels. The changes of vitamin E status and antioxidant enzyme activity by CLA were also observed in mice when fed with high-fat diet. However, this could be due to the reduction of body fat (where the vitamin E stored) and the oxidative stress increased by high dietary fat.