The effect of resveratrol on DSS-induced colitis in mice

• Main Authors: Wei-chih Lai, 賴韋誌
• Other Authors: none
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/6e82v9

碩士 === 嘉南藥理科技大學 === 營養與保健科技研究所 === 100 === Our previous studies indicated that dietary oxidized frying oil (OFO) resulted in deterioration of dextran sulfate sodium (DSS)-induced colitis in mice. However, mechanisms for these effects remain unknown. The purpose of this study was to investigate the effect of trans-resveratrol supplementation on DSS-induced colitis in OFO-fed mice. In this study, to elucidate the possible mechanisms underlying OFO effects, we focus on three aspects including antioxidant-prooxidant system, inflammation and colonic mucosa function. 7-wk-old female C57BL/6 mice (n=40) were used in this study. They were acclimatized in our animal house were fed powder diets and water ad libitum. There were randomized into three dietary groups, including C group (15% fresh soybean oil), O group (15% OFO) and R group (15% OFO+10 mg trans-resveratrol/kg diet). After the 6-wk feeding period, mice were treated by 2% (w/v) DSS administered in drinking water for 5 days, followed by providing DSS-free water for 2 days. After the 12 h fasting period, mice were sacrificed with carbon dioxide asphyxiation. Blood, liver and colon were collected and assessed for associated parameters, such as inflammatory cytokines, antioxidant defense system, and colonic mucosal function. Our results showed that body weight gain and feed efficiency were significantly decreased in the O and R groups than in the C group. Mice exposed to DSS to induce acute colitis, disease activity index (DAI) were significantly increased in the O and R groups than in the C group. According to DAI results, we found no significant preventive effect of resveratrol on OFO-worsen colitis. Serum total antioxidant status (TAS) levels were significantly increased in the O and R groups than in the C group. We used serum haptoglobin (Hp) to assess the state of systemic inflammation. We found that O group was significantly elevated compared with C group, but had a significantly decrease in R group compared with O group. In addition, serum alanine transaminase (ALT) activity in the O and R group had a increase compared to the C group. Thiobarbituric acid reactive substance (TBARS) in the liver of the O group and R group were higher than those of C group. That indicated that TBARS were significantly increased in OFO-fed mice. The level of vitamin C was significantly higher in R group than that in C group. Glutathione S-transferase (GST) and catalase activities in the O group and R group were significantly increased compared to the C group, superoxide dismutase (SOD) and Se-Glutathione peroxidase (Se-GPX) activities in the O group was significantly decreased compared to the C group, the activities of Se-GPX was significantly lower in C group than that in R group. Colonic myeloperoxidase (MPO) activities were significantly decreased in the O group and R group. We conjectured that had distinctive effect of OFO on MPO expression. In the mRNA expression of liver, results showed relatve amounts of COX-2 and IL-6 mRNA in R group were decreased as compared with C group and O group, the expression of IL-1β mRNA were significantly lower in the R group than in the C group. Trans-resveratrol had a significant decrease on hepatic proinflammatory cytokines expression in DSS-induced colitis in mice. Results showed that colonic mRNA expression were no significant differences existed among 3 groups, including matrix metalloproteinases (MMP3), MMP9, mucin2 (MUC2), trefoil factors-3 (TEF3) and hairy enhance of split-1(HES1). In conclusion, trans-resveratrol had no marked inhibitory effect on OFO-worsen DSS-induced colitis in mice. We speculated that (1) the dosage of trans-resveratrol was too low, (2) the adverse effect of OFO might be not due to either the antioxidant imbalance or aggravating inflammation. In addition, the lowing effects of trans-resveratrol on pro-inflammatory cytokines were significant in liver than in colon.