Anti-inflammatory and antioxidative effects of Barley grass, Garcinia mangostana , and natural vegetable fruit enzymes

• Main Authors: Ching-Yi Chen, 陳經宜
• Other Authors: Kuan-Han Lee
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/xkk235

碩士 === 嘉南藥理科技大學 === 藥物科技研究所 === 100 === 　　The primary symptom of Osteoarthritis is pain, which is one of the most common chronic diseases in the world as well. Traditional painkillers (including steroid and non-steroid anti-inflammatory drugs) can cause side effects under long term use, such as peptic ulcer, lower limb edema and impairment of kidney. Since the side effects of drug treatment are quite disturbing annoying, the study of natural substances that which can fight joint inflammation appears especially important. This study used: (a) barley grass extract, (b) Garcinia mangostana extract, and (c) natural vegetable fruit enzymes, or their mixtures, applied with 3T3 mouse fibroblast or cell supernatant, and proceeded with the analysis of enzyme activities of matrix metalloproteinases and hyaluronidaseas as well as DPPH free-radical scavenging capacity. 　　The experimental results show that the ability of inhibition towards MMP-2, MMP-9 and of MMP-13 was obvious by treating 1.0% mangosteen water extracted for 24 and 48 hours, whereas 2.5% of Garcinia mangostana extract showed apparent inhibition on the activity of MMP-2, MMP-9 and MMP-13 after 24 h treatment (in the 24 and 48 h treatment). Comparatively speaking, barley grass extract and natural vegetable fruit enzymes , at concentrations of 5.0% and 10.0%, show unapparent inhibition on MMP-2, MMP-9 and MMP-13 in 24 and 48 h after treatment. 1.0% of the mangosteen water extract of the processing time of 24 h, completely inhibited the activity of hyaluronidase, showing its high rejection; a final concentration of 5.0% for a long time Secretary natural vegetable fruit enzymes water extract of the processing time 24 h has little effect of inhibition of hyaluronic acid; nevertheless, when the concentration was increased to 10.0% and the time to 48 h, a strong effect of inhibition of hyaluronidase has been observed. barley water extract at a final concentration of 5.0 and 10.0% for hyaluronidase has little inhibition effect at 24 h; however, with 48 h compared with the control group which does not have the ability to inhibit the role of hyaluronidase. 　　Antioxidant experimental result indicates that the DPPH free-radical scavenging rate of 200 μg/mL of Garcinia mangostana is quite high, i.e., up to 83.0%; while that of 400 μg/mL of barley grass is 17.0%; and 400 μg/mL of natural vegetable fruit enzymes showed no apparent DPPH free-radical scavenging capability. 　　Garcinia mangostana possesses the ability to inhibit MMPs and hyaluronidaseas, as well as the anti-inflammatory and antioxidative properties based on the above experimental data. In conclusion, Garcinia mangostana is an active ingredient that can be used for the treatment of joint inflammation.