The flavonoid-rich extract from Nelumbo Nucifera leave inhibited complications of type II diabetes mellitus

• Main Authors: Yun-Chia, 洪云佳
• Other Authors: 王朝鐘
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/ym5t6a

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 100 === Type II diabetes mellitus (type II DM) patients have insulin resistance, which leads glucose fail to transport for energy metabolism and causes hyperglycemia. Hyperglycemia induces the generation of reactive oxygen species (ROS) and enhances oxidative stress. Overproduction of free radicals causes various diabetic complications including hyperglycemia, hypertension, hyperlipidemia and other. Diabetic vascular disease is the major lethal reason in type II DM patients. In this study, low dose streptozocin (STZ) was administrated to induce type II DM in HFD-fed SD rats. After the STZ induced, the effect of Nelumbo Nucifera leave extract (NLE) was observed in type II DM rats. Our results showed that NLE induced AMPK phosphorylation, increased insulin secretion, promoted anti-oxidant enzyme activity, and through regulated MAPKs and Akt protein expression to reduce fatty liver formation, ameliorate renal injury and inhibit vascular disease in type II DM rat. In cellular experimental, NLE inhibited high glucose-induced proliferation and migration in VSMCs. Furthermore, NLE ameliorated insulin signal, enhanced anti-oxidant enzyme activity, and reduced MAPKs, Akt and inflammatory factor expression. In brief, NLE attenuated diabetic complications in type II DM rats and inhibited HG-induced proliferation and migration in VSMCs. These data suggested that NLE might provide alleviative effects against diabetic deterioration.