The effects of medium chain fatty acids on palmitate-induced inflammatory responses in C2C12 skeletal muscle cells

• Main Authors: Ya-Fang, 陳雅芳
• Other Authors: Te-Chung Liu
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/gs24t8

碩士 === 中山醫學大學 === 營養學研究所 === 100 === Inflammatory response refers to the protection of body tissues from appearance of stimulating factor such as injury, foreign object intrusion and oxidative stress. However, chronic inflammation plays an important role in pathogenesis of many diseases, including diabetes, fatty liver and atherosclerosis. It was reported that exposure of C2C12 skeletal muscle cells with the saturated fatty acid palmitate (16:0) could increase expression of proinflammatory cytokines Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) to induce inflammation which may be related to insulin resistance. Medium-chain fatty acids (MCFA) are saturated fatty acids, carrying carbon number between 6-14. It is often used in clinical nutrition intervention for critically ill patients to instead of long chain fatty acids to improve fat utilization for critically ill patients. The effect of MCFA on inflammation still do not understand so in this study, we explore the role of MCFA, lauric acid (C12:0) and mystiric acid (C14:0) in C2C12 Cells. Although the MCFA, lauric acid and mystiric acid slightly induced COX-2, IL-6 and TNF-α expression, but induced lower than palmitate.Moreover, co-incubation of cells with palmitate and lauric acid or mystiric acid reversed palmitate-induced PKC-θ phosphorylation, NF-κB activation and COX-2 expression. These findings may suggest that MCFA lauric acid and mystiric acid could decrease palmitate-induced COX-2 expression through inhibition of PKC-θ/IκBα/NFκB pathway.