Studies of liquid spawn culture for Pleurotus eryngii

• Main Authors: Chih-Hao Chen, 陳志豪
• Other Authors: Hsiao-Sung Chan
• Format: Others
• Language: zh-TW
• Published: 2011
• Online Access: http://ndltd.ncl.edu.tw/handle/38026338057525449073

碩士 === 朝陽科技大學 === 生化科技研究所碩士班 === 100 === Abstract‏ Pleurotus eryngii is an edible mushroom it has a thick, meaty white stem and a small tan cap. The mushroom has a good shelf life but an effective cultivation method is needed to enhance the biomass of this fungus for commercialization. Hence, the present work describes the use of different additives (sugars and nitrogen source) and treatments to enhance the biomass of commercially used P. eryngii ESPS strain for liquid spawn. The CS medium was used as basal medium and mycelium were grown in submerged culture conditions. The initial inoculums were obtained from the mycelia harvested from the petri dish and homogenized at 15,000 rpm. Approximately, 10 % (v/v) homogenized mycelia was used as initial inoculums and transferred to the 250 mL Erlenmeyer flask containing 50 mL of liquid CS medium. Effect of different monosaccharides, disaccharides and organic nitrogen source were examined one by one on mycelium growth. The significant growth response was observed with different carbon source (sucrose and glucose) and nitrogen source (yeast extract and peptone). Response Surface Methodology (RSM), was used to investigate the effect of these additives on mycelia growth. With the goal to achieve maximum mycelium production four components were tested in the process of media optimization. According to central component design and mathematical calculations the optimal concentration of sucrose, glucose, yeast extract and peptone were 24.8 g/L, 11.1 g/L, 21.8 g/L and 6.8 g/L respectively. The prediction of maximum mycelium production was 9.48 g/L, which coincides with the shaker-flask mycelium production 10.19 g/L. Based on results obtained from RSM, the experiment was extended to 6-L stirred tank reactor (STR) and mycelium were grown for eight days. About, 20 mL of this inoculum was poured as seed in bag cultivation as well as in sawdust. The high growth rate and fruiting body production were obtained in bag cultivation than the sawdust cultivation. An increase of 1.2 fold of the fruiting body production was obtained with bag cultivation (331.67 ± 7.55 g) compared to sawdust spawn (276.67 ± 6.75 g). The efficiency of cultivation was increased to 94.22% in bag cultivation compared to 78.60% in the control group. The present investigation on media optimization can be used for higher biomass production of P. eryngii ESPS strain and may be implemented to other Pleurotus strains.