Development of Wound Healing Films Using Bacterials Cellulose/Collagen/Gelatin Composites

• Main Authors: Wen, Chunchieh, 温竣傑
• Other Authors: Wu, Janeyii
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/92965392564987255388

碩士 === 大葉大學 === 生物產業科技學系 === 100 === 　　Collagen is an insoluble fibril protein with normaly exists in vertebrate about 30%. Collagen is a high biocompatible material with a wide variety of medical, cosmetic and wound dressing applications. The purpose of this research is aimed to develop (1) extracted collagen and gelatin from Chanos chanos scale and characterized them, (2) then use collagen and gelatin cross linking with BC by glutaraldehyde, and the products BCC (bacterial cellulose-collagen) and BCG (bacterial cellulose-gelatin) were culture with 3T3-L1, HSFs and CHO-K1 to investigate cell proliferation, (3) use DBD (dielectric barrier discharge) atmospheric plasma treatment BC surface for hydrophilic. The description of this is divided into three sections. 　　The first section: Collagens and gelatins were extracted from waste scale of Chanos chanos by organic acid. The results showed that the highest yield of the collagens were 10.23 mg/g scale by citric acid. The molecular messure of the collagen subunits were about 130 kDa for α1 and 120 kDa for α2, respectively. FTIR (fourier transform infrared spectroscopy) and EDS (energy dispersive spectrometer) analysis indicated that the extracted product was a collagen. The second section: MTT (3-(4, 5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium) assay indicated that the best GTA concentration HSFs and CHO-K1 cultured on BC membrane cross linking with different concentration of glutaraldehyde. These results show BCC and BCG can increase cell adherent, proliferation and good morphology using fluorescence stain. Use SEM and AFM analysis indicated that BCC and BCG membrane were coated with collagen and gelatins. 　　The third section: The purpose was to study the influence of the main operating parameters on the BC surface modification, i.e. carried gas, gas flow rate, power supply, plasma jet distance, treat passes and power frequency. It was found that the plasma treatment is an efficient method used for improved hydrophilic of the BC membrane surface.