| Summary: | 碩士 === 逢甲大學 === 自動控制工程所 === 100 === A centrifugal microfluidic fluorescence detection system and a multifunctional biochemical detection array are developed with the functions of real-time dynamic imaging, pneumatic control and wireless transmission. The developed system can be controlled by integrating pneumatic force and centrifugal force. The flexible pneumatic valves combined with passive capillary valves have been applied in automatic control mechanisms to perform microflow functions of successional drive-in, separation and blending. The modulation of flexible pneumatic device is used to single or two-directional centrifugal control that can reduce sample quantity without complicating disk structures for increasing inspection operation function. A biochemical detection array is designed for detecting microfluidic biochemical reaction using the luminal-based fluorescence agent that is applied to determinate the concentrations of vitamin B1 and arsenic in rices. The luminol, hydrogen peroxide and luminol-KMnO4 chemiluminescence system has used to achieve vitamin B1 and arsenic measurements of rices in the ranges of 0.05~8 ppm and 0.002~4 ppm respectively. The vitamin B1 response is linear with the sensitivity 2.388 ppm V-1. The response of arsenic is nonlinear which three-piecewise linear approximation is processed. The sensitivities of arsenic are -0.017, -0.050, and -2.974 ppm V-1 for the ranges of 2~14×10-3, 15~90×10-3, 0.3~4 ppm, respectively. Experimental detection has been executed in heavy metal concentration and water absorption of rices. Experimental results show operation efficiency of fast and accurate analysis that can be used in food ingredients analysis synchronously.
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