The Effects of Selenium Compounds on Proteomic Expression and Oxidative Stress in Aged Mice and Rat Testis.

• Main Authors: Pei-Ying Chiang, 江沛瑩
• Other Authors: Shyh-Mirn Lin
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/49307341852415525772

碩士 === 中華醫事科技大學 === 醫學檢驗生物技術系碩士班 === 100 === Increase with age, testicular function, such as the manufacture of sperm and hormonal effects reduce the increase in its causes and testicular oxidative damage. Contain high levels of selenium in the testis, many studies have shown that selenium anti-inflammatory, anti-tumor effects, its mechanism of action and antioxidant capacity. The purpose of this study was to investigate the two forms of selenium supplement on aging mice testis oxidation state and proteomics. 64 weeks of BALB / c male mice were randomly divided into three groups according to body weight, and were fed to experience feed: control group (OC), inorganic selenium group (SE; 0.5 mg/kg diet), and organic selenium group (SY; 0.5 mg/kg diet). We use 6-week-old young rats, as the young control group (YC). Seven weeks after feeding, analysis of testicular tissue fluid homogenate antioxidant enzyme activity was found in the oxidative stress of the OC group which was significantly higher than the YC group; therefore, we found that selenium supplementation can improve the increased oxidative stress. In the testis protein changes of two-dimensional gel electrophoresis, we found seven protein spots for the OC group was notably higher than the YC group, and the SE group with SY group was obviously lower than the OC group identified by identification by mass spectrometry identity of their protein, which contains 78 kDa glucose-regulated protein (GRP78). These proteins are associated with aging; through selenium supplementation they can reduce the increase due to aging. Then we analyze 78 kDa glucose-regulated protein (GRP78) with western blot for further and discover that the expression of GRP78 in the OC group testicles is lower than the YC group, while the complement of different types of selenium, the performance showed a significant increase. Additionally, we adopt 79-week-old SD male rats randomly divided into five groups according to body weight, fed with experience feed: control group (OC), inorganic selenium group (LSE; 0.5 mg/kg diet、HSE; 2 mg/kg diet), and organic selenium group (LSY; 0.5 mg/kg diet、HSY; 2 mg/kg diet). At the other side, 14-week-old young rats, as the young control group (YC). Twelve weeks after feeding, analysis of testicular tissue fluid homogenate antioxidant enzyme activity was found in the oxidative stress, western blot analysis of GRP78 expression in the testes. The γ-GTP activity was measured; we found the rats of the results compared to the results of the analysis of the mouse testis, their tendencies are the same. This study shows that, given selenium supplements can improve the antioxidant capacity of the testes of aging, and affect the performance of testicular protein. This result can be used as the reference to prevent the degradation of testicular function.