| Summary: | 碩士 === 高雄醫學大學 === 牙醫學研究所 === 100 === Objectives : Studies have shown that genetic factors can account for a large part of the variation seen in periodontal disease expression in humans. CD14, a glycoprotein localized on the cell surfaces of myeloid cells, functions as a pattern recognition receptor for various bacterial products, such as LPS. Therefore, the aim of the present study was to determine whether the SNP at position -159 in the promoter region of the CD14 gene is associated with periodontal disease status in a Chinese Han population.
Material and method :A toatal of 270 subjects with CP(N=122), AgP(N=84), and periodontal health(N=64) volunteered to participate. Data concerning smoking, drinking and betal quid chewing were obtained by interviewing the subjects in association with the clinical examination. Genomic DNA was extracted from blood. Genotypes of CD14 -159 C/T were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The data were analyzed by χ2 test, one-way ANOVA and multiple logistic regression analyses. The risk for periodontitis associated with genotypes was calculated as the odds ratio (OR).
Result : The genotype distribution of CP patients showed that 13.9% were CC, 53.3% were CT, and 32.8% were TT. AgP patients showed that 11.9% were CC, 47.6% were CT, and 40.5% were TT. These were not significantly different from the distribution in control subjects(15.6% were CC, 42.2% were CT, and 42.2% were TT). Adjusted variables such as sex, age, smoking, drinking, and betal quid chewing, the prevalence of the CT+CC genotype compared with the TT genotypes was significantly lower in CP of non-smoking group. (Adj. OR: 0.41, 95% CI : 0.16 ~ 0.98)
Conclusion: The results of this study suggested that the TT genotype of the CD14 -159 C/T polymorphism seems to be associated with an increased risk for chronic periodontitis in a Taiwanese population. The TT genotype effect was overwhelmed by smoking habit.Further research is necessary to prove the polymorphisms to have a functional effect.
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