| Summary: | 碩士 === 高雄醫學大學 === 醫學檢驗生物技術學研究所 === 100 === 英文摘要
A diabetes epidemic has emerged during the 20th century. The prevalence of diabetes for all age groups was estimated to be 2.8％ in 2000，and this number will increase to 4.4％ by 2030. Alterations in trace elements have been reported in a number of diseases. Patients with diabetes mellitus are inclined to result in the imbalance of trace elements and elevated oxidative stress markers. Trace elements are well known to play an important role in the maintenance of health. Zinc is essential for normal insulin production, and plays a central role in cellular protection against apoptosis and oxidative stress. Copper is an essential trace element for enzyme systems catalase, superoxide dismutase and cytochrome oxidase and its deficiency can lead to a variety of nutritional and vascular disorders. Vitamin D through its active form 1, 25-dihydroxyviyamin D, is essential for intestinal calcium absorption and plays a central role in maintaining calcium homeostasis and skeletal integrity. Vitamin D deficiency may be also related to cancer, cardiovascular disease, diabetes and multiple sclerosis in human. In this study, trace elements and vitamin D in body fluids of type 1 diabetic patients were evaluated. We further explored the possible relationship between trace elements, vitamin D and parameters (duration of the disease, HbA1c, gender, BMI value) of diabetic patients.
Part I: The aim of this study was to explore the serum copper and zinc levels in type 1 diabetic patients by flame atomic absorption spectrometry. Zinc level was significantly lower than controls, but they failed to reach statistical significance. Copper levels was significantly higher in type 1 diabetic patients when compared with the control groups (p＜0.001). No significant differences were observed while analysimg the relationship between five parameters and trace elements. In summarys, there are no significant correlation between trace elements (Zn﹐Cu) levels and duration of diabetes, HbA1c, gender, BMI values in type 1 diabetic patients.
Part II: There were controversial results of serum vitamin D in type 1 diabetic patients. There were still very rare previous literatures concerning serum vitamin D level in diabetic patients by using liquid chromatography tandem mass spectrometry. We found that our type 1 diabetic patients had significantly higher serum 25(OH)D and 25(OH)D3 levels than that of control groups. There were no significant difference of serum 25(OH)D2 levels in type 1 diabetes and control groups. Our study revealed that 41.5% of type 1 diabetes male patients were 25(OH)D insufficient and 7.3% deficient. In type 1 diabetes female patients, 13.3% were 25(OH)D insufficient and 36.7% were 25(OH)D deficient. There were no correlation between 25(OH)D levels and HbA1c in type 1 diabetes patients. There were also no correlation between 25(OH)D levels and duration of the disease in type 1 diabetes patients. There were no correlation between serum copper, zinc levels and serum 25(OH)D levels in type 1 patients, respectively.
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