| Summary: | 碩士 === 國立中興大學 === 環境工程學系所 === 100 === The aims of this study were to measure the background level of 17B-estradiol (E2) and naphthalene quinone (NPQ)-derived protein adducts in human albumin (Alb) derived from female breast cancer patients (n=67) in central Taiwanese women and to explore the relationships between these adducts with risk factors of breast cancer (Age, Body Mass Index, Single Nucleotide Polymorphism). Results from the analysis indicated that Alb adducts of estrogen quinones, including E2-2,3-quinone (E2-2,3-Q-4-S-Alb) and E2-3,4-quinone (E2-3,4-Q-2-S-Alb) were detected in all subjects (n=67) at average levels were 393 (263) and 681 (330) pmol/g, respectively. In addition, the average levels of cysteinyl adducts of 1,2-NPQ and 1,4-NPQ on human serum albumin were estimated to be 237 (130) and 78.3 (47.5) pmol/g, respectively. Levels of 1,4-NPQ-Alb correlated significantly with those of 1,2-NPQ-Alb (r=0.420, p < 0.001). Similarly, levels of E2-2,3-Q-4-S-Alb correlated significantly with those of E2-3,4-Q-2-S-Alb (r=0.707, p < 0.001). When comparing these data with our previous study (n=85), we noticed that among those breast cancer patients with age under 50 and BMI less than 27, BMI inversely associated with E2-3,4-Q-2-S-Alb (r=-0.241，p<0.05). Additionally, levels of 1,2-NPQ-Alb positively correlated with age (r=0.266，P<0.001). Levels of 1,2-NPQ-Alb in patients with BMI over 27 were 30% higher than patients with BMI under 27. We noteced that the ratio of E2-3,4-Q-Alb and E2-2,3-Q-Alb derived adducts in breast cancer patients were ~4 fold greater than those of controls. This evidence suggests that the cumulative body burden of E2-3,4-Q is a significant indicator of the risk of breast cancer.
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