| Summary: | 碩士 === 國立中興大學 === 生命科學系所 === 100 === Fatty liver disease has been defined as intrahepatic triglyceride (IHTG) content ＞5% of liver volume or liver weight in animal or human being. Fatty liver is commonly associated with obesity, insulin resistance, and diabetes. It is estimated that about 16-23% of population exhibits the syndrome of the non-alcoholic fatty liver disease (NAFLD) in world wide. Severe fatty liver is sometimes accompanied by steatohepatitis or develop hepatocellular carcinoma. To date, there is still no effective therapy to treat NAFLD, so the focus of current investigations has been on the development of effective components or functional products with bioactivities. In this study, we used leptin gene knockout ob/ob mice as a NAFLD animal disease model. Six-week-old ob/ob mice were administered with fermented product (140 mg/kg of body weight per day) for four weeks. Four experimental groups were designed as followed: (1) ob/ob mice treated with ddH2O group, (2) ob/ob mice treated with fermented product group, (3) WT mice treated with ddH2O group, and (4) WT mice treated with fermented product group (n=6). After oral administration, fermented product treatment improves fatty liver syndrome by inhibiting liver lipogenesis gene expression, and decreasing the contents of cholesterol and triacylglyceride (TG) in liver (p<0.05). In addition, the result showed that treatment with fermented product has significantly reduced oil drops in liver tissue examed by H&E stain and oil red stain. Furthermore, we found that fermented product treatment with ob/ob mice markedly decreased the expression of sterol regulatory element-binding protein-1 (SREBP-1), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) gene that was an effect on reducing lipogenesis gene (p<0.05). Fatty acid-riched HepG2 cell culture treated with the peptide fractions from fermented product also decrease lipogenesis gene expression. We speculated that fermented product improve the NAFLD throught the inhibition of lipogenesis pathway. In conclusion, our data suggested that peptides derived from fermented product may have potentials for clinical applications in the prevention or treatment of NAFLD.
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