Effects of long-term leptin administration on homocysteine transmethylation and transsulfuration

• Main Authors: Shih-Ting Huang, 黄詩婷
• Other Authors: En-Pei Isabel Chiang
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/86818428952159774920

碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 100 === Our previous study demonstrated novel finding of a direct promoting effect of high cellular insulin or glucose exposure on homocysteine remethylation, adoMet synthase activity, and adoMet synthesis. We have also provided new evidence indicating that when hepatic tissue is exposed to elevated insulin or glucose, the cellular methylation balance is altered. Hyperleptinemia is commonly seen in humans suffering from metabolic syndrome or type II diabetes; and leptin concentration has been suggested as an independent predictor of type II diabetes in men. However, the impact of path physiological elevations in leptin on homocysteine metabolism is not fully illustrated. The objective of the study was to investigate the impact of elevated leptin on homocysteine metabolism and methyl group kinetics in vivo. Effects of long-term leptin administration on homocysteine transmethylation and transsulfuration metabolic fluxes were investigated in mouse models using stable isotopic tracers and gas chromatography/mass spectrometry. Homocysteine remethylation and transsulfuration metabolic fluxes were investigated using stable isotopic tracers and gas chromatography/mass spectrometry. The methylation status was determined. Our preliminary in vivo study indicated that long-term leptin administration altered homocysteine metabolism in a tissue specific manner.