Microarray Dataset Analysis of the Cav3.2 T-type Calcium Channel Knockout Mouse Strain

• Main Authors: I-Lin Ho, 何宜霖
• Other Authors: 蔡孟勳
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/90652734788557410845

碩士 === 國立中興大學 === 資訊管理學系所 === 100 === Different brain areas are responsible for specific functions of learning and memory. Among them, the hippocampus plays an important role in forming spatial, episodic and long-term memory. The α1H T-type Ca channel (Cav3.2) triggers many intracellular biochemical events, including muscle contraction, gene expression or hormones and neurotransmitter secretion. It has been demonstrated that the α1H T-type Ca2+ channel (Cav3.2) is highly expressed in the hippocampus and where it plays a critical role in hippocampal‐dependent learning. This thesis analyzed genome-wide expression microarray datasets obtained from the hippocampi of either naive or trace fear conditioned-α1H T-type calcium channel knockout mice for changes of transcriptome. First, paired-samples t-test was adopted to confirm whether there were significant difference of gene expression before and after training exist. Next linear regression analysis was applied to single out targeting genes which displayed significant difference in gene expression. After that, hierarchical clustering was implemented to find the cluster of NA (non-available) genes whose biological functions have not been confirmed biologically. The target genes were then put into the genetic information system for a comparison of their association with the development of the hippocampus in an attempt to find out whether they affected learning and memory. The results indicated that the right hippocampus of naive α1H T-type calcium channel knockout mice showed significant number of differentially expressed genes. The significant number of differentially expressed genes in the left hippocampus can be reduced to close to Wild Type group. This thesis research will provide a promising direction for further functional studies of the filtered targeting genes.