The Apoptotic Effect of Cisplatin and Taxol Combined with Cordycepin on MA-10 mouse Leydig Tumor Cells

• Main Authors: Pei-JungChen, 陳姵蓉
• Other Authors: Bu-Miin Huang
• Format: Others
• Language: en_US
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/35727373988082955119

碩士 === 國立成功大學 === 細胞生物及解剖學研究所 === 100 === The causes of Leydig tumor cell still remain unknown. Recently, chemotherapies are not limited to single treatment anymore, and evidence suggests that different drug combinations are applied in patients with positive responses. Besides, the effects of anticancer drugs generally cause cell morphological changes and cell cycle arrest at different phases to induce cell apoptosis. Previous data in our laboratory have shown that cordycepin could induce MA-10 cell apoptosis by activating caspase-3, -8 and -9. Consequently, we investigated the possible additive and/or synergistic effect in apoptosis by cisplatin and/or taxol combined with cordycepin in MA-10 mouse Leydig tumor cell line. Results showed that MA-10 cells exhibited the apoptotic features, including cellular rounding-up and membrane blebbing, after treating with cordycepin, taxol, cisplatin, or cordycepin plus cisplatin and/or taxol for 24 hours. In MTT assay, drug alone or in combination treatments diminished MA-10 cell viability in a dose-dependent manner, and there were additive effect in various drug combinations, except for cisplatin plus taxol treatment. In flow cytometry assay, the treatments of cisplatin alone or cisplatin plus taxol induced cell arrest at G2/M phase without significant change in sub-G1 phase. However, other treatments displayed cell accumulation at sub-G1 phase. Moreover, the mechanism of apoptosis was detected by Western blotting. Data showed that caspase and MAPK and p53 signal pathways could be induced under the various treatments. Taken together, we found that cisplatin and/or taxol combined with cordycepin could induce an additive effect of apoptosis in MA-10 cells.