| Summary: | 碩士 === 國立成功大學 === 體育健康與休閒研究所 === 100 === Purpose: To investigate the effects of endurance exercise (EXE) and momordica charantia (MC) contained diets on bone qualities in growing rats. Methods: Seventy-two male SD rats (7 weeks old) were randomly divided into six groups, which were the 0%MC, 5%MC, 8%MC, 0%MC+EXE, 5%MC+EXE, and 8%MC+EXE groups (n=12 for each). After six-week experimental period, all animals were sacrificed. Blood and bone samples were collected, prepared and stored for various analyses. Two-way (MCEXE) ANOVA was used for statistical analysis (α=.05). Results: In exercise main effect, exercise animals showed significantly lower body weight (p〈0.05), lower epididymis fat tissue weight (p〈0.05) and serum leptin (p〈0.05). In histomorphometry, the main effect analysis of MC showed that bone volume ratio and trabecular separation (p〈0.05) of the rats feed with MC contained diets were significantly lower and higher, respectively, than the non-MC treated animals. In addition, interaction reached significant level in trabecular thickness that the 0%MC group was significant higher than the 5%MC and 8%MC groups while no difference was shown among three exercise groups; suggesting exercise’s efficacy in counteracting MC’s negative impact on trabecular bone. In bone cell analyses, MC and EXE respectively showed their effects in down-regulating bone formation and bone resorption. In biomechanical analyses, MC and EXE caused body weight decrement seemed to further reduced several size-related mechanical properties. Nevertheless, exercise somewhat modified MC’s related negative effects on bone. In tissue size measurements, MC fed animals showed lower cross-section area, cortical area and cortical thickness while exercise animals only showed lower values in cortical area. Conclusions: There seemed to be negative effects of the MC supplement diets on bone qualities. Endurance exercise showed some modifications on MC’s effects in bone material.
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