| Summary: | 碩士 === 國立成功大學 === 環境醫學研究所 === 100 === Colorectal cancer (CRC) is the third most common cause of cancer death in the world and also is a major cause of worldwide morbidity and mortality. In recent studies, plenty of evidences indicated that tyrosine-phosphorylated (pTyr) proteins were involved in malignant tumor cell invasion and metastatic regulation. In this study, the goal is to get a better understanding of metastasis-associated signaling pathway underlying human colorectal cancer. We identified the differentially expressed pTyr proteins from the same patient of primary colorectal adenocarcinoma SW480 cell and lymph node metastasis SW620 cell are compared. By performing adhesion assay and Western blotting of FAK, the data showed that SW620 metastatic ability were better than SW480. Using our analytical strategy for filtering out the potential pTyr peptide signals, we found the signals of 365 m/z values to be identified 280 pTyr peptides containing 287 pTyr sites from 261 proteins. Finally, the 103 differentially expressed pTyr proteins (p〈0.05) were found in the two cells using the label-free quantitative analysis. From this list of site, we extracted two novel consensus sequences and four known motifs for specific kinases and phosphatases including EGFR, Src, ALK, and SHP1. Moreover, 10 target pTyr proteins were with higher level in SW480 cells while 3 with higher levels in SW620 cells. After interactome analysis by Metacore software, 10 of 13 target pTyr proteins are interacted with SOX2,FKHR,E2F1 metastasis or cell-cycle genes. Above this information may provide us a direction to understand CRC metastasis and those pTyr protein targets identified in this study may provide novel information to not only mechanistic aspects of colorectal cancer metastasis but also drug targets for hampering the cancer metastatic processes.
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