| Summary: | 碩士 === 國立暨南國際大學 === 電機工程學系 === 100 === Uric acid is a product of purine metabolism, and it is widely distributed in human blood and urine. High level of uric acid could lead to many diseases, such as gout or arthritis. Therefore, determination of uric acid was important for diagnoses patients and medical studies. This study develops a simple, innovative and high accuracy of the uric acid biosensor array (UBA) to accurately detect uric acid levels for human blood. A low cost, miniaturization of amperometric UAB fabricated from screen printing carbon electrodes and immobilized uricase on working electrodes.
In this study, we tested the impact of the number of working electrode and shelf－life on the uric acid biosensor. The UBA showed a good linear response range for determination of uric acid at 0.7 V (versus reference electrodes) and the response time was 310s reaching steady state current value. The relative standard deviations for determination of 0.8mM uric acid standard solution for 1X1 uric acid biosensor, 1X2 UBA and 1X3 UBA was 5.98, 4.94 and 5.38%. Besides, the 1X3 UBA had the best signal to noise ratio for determination of uric acid. Finally, the uric acid biosensor was stored at 4oC, and still maintained 87.9% from the original activity after 60 days.
Our results conclude that development of an electrochemical biosensor to detect uric acid by increasing number of electrodes increase the signal to noise ratio. And it is capable to precisely determining blood uric acid levels in the range of normal physiological and pathological regions. Overall, the UBA we design using increase number of electrodes was benefit in greater sensitivity and inexpensiveness.
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