| Summary: | 碩士 === 國立體育大學 === 運動科學研究所 === 100 === Exercise training has been shown to to improve aging liver function and antioxidant activity. The effects of swimming exercise on anti-aging liver are largely unclear. Objective Therefore, the aim of the present study was to determine whether 12-week swimming exercise can protect the liver, and if so, to investigate the underlying liver senescence mechanism. Methods Twenty-three male Sprague-Dawley rats were divided into the following four groups: (1) control group (C: n = 6) , in which aging was not induced, (2) aging group (A: n = 6) , in which aging was induced but the rats were not subjected to exercise , and (3) swimming group (S: n = 5), in which aging was induced but the rats were not subjected to aging , and (4) the aging- swimming group (AS: n = 6), in which aging was induced and the rats were subjected to swimming exercise. D-galactose (150 ml/kg) was injected into the abdominal cavity for 12 weeks to induce aging. Rats were subjected to swimming exercise 5 days a week for 12 weeks, and 60 min a day. Clinical changes, including weight change and epididymal fat, serum biochemical parameters ,histopathology examination and apoptosis, were recorded. Liver cellular senescence was assessed by β-galactosidase (β-gal) activity and the expression of p53, p21 , and pRb. Levels of tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in liver were also examined. Results A group against body weight and epididymis fat gain (p < 0.05) . The protein levels of p53, p21, and pRb were significantly lower in the AS groups than in the A group (p < 0.05).β-gal activity was significantly higher in the A and AS groups than in the C and S groups (p < 0.05).
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Levels of TNF-α and IL-6 were significantly decreased in the AS and S groups ( p < 0.05). Besides, the A group was higher inflammatory response by H&E stain assay. Conclusion Swimming exercise for 12 weeks may suppress cell senescence and regulation of immune and inflammatory responses in the liver of induced aging rats.
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